Evidence map›Paper›PMID 27618147›Full record

ArticleProteins2016

Substrate specificity characterization for eight putative nudix hydrolases. Evaluation of criteria for substrate identification within the Nudix family.

Vi N Nguyen, Annsea Park, Anting Xu, John R Srouji, Steven E Brenner, Jack F Kirsch

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Proteins, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed.

  1. Article
  2. Molecular Dialog ofInternational journal of molecular sciences · 2025
    Review
  3. Article
  4. Article
  5. Article
  6. Article
  7. Article
  8. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Vi N NguyenMolecular and Cell Biology Department, University of California, Berkeley, California, 94720.
Annsea ParkMolecular and Cell Biology Department, University of California, Berkeley, California, 94720.
Anting XuGraduate Program in Comparative Biochemistry, University of California, Berkeley, California, 94720.
John R SroujiMolecular and Cell Biology Department, University of California, Berkeley, California, 94720.
Steven E BrennerMolecular and Cell Biology Department, University of California, Berkeley, California, 94720.
Jack F KirschMolecular and Cell Biology Department, University of California, Berkeley, California, 94720.

Funding

Protein function prediction by statistical phylogenomicsR01GM071749 · NIGMS · UNIVERSITY OF CALIFORNIA BERKELEY · PI BRENNER, STEVEN E · 2007 to 2010
$1.4M
NIGMS NIH HHS R01 GM071749
6 · The paper itself

Abstract

The nearly 50,000 known Nudix proteins have a diverse array of functions, of which the most extensively studied is the catalyzed hydrolysis of aberrant nucleotide triphosphates. The functions of 171 Nudix proteins have been characterized to some degree, although physiological relevance of the assayed activities has not always been conclusively demonstrated. We investigated substrate specificity for eight structurally characterized Nudix proteins, whose functions were unknown. These proteins were screened for hydrolase activity against a 74-compound library of known Nudix enzyme substrates. We found substrates for four enzymes with k

Indexed as

Adenosine Diphosphate RiboseBacillusBacterial ProteinsCloning, MolecularClostridium perfringensDeoxyadenine NucleotidesDeoxyguanine NucleotidesDinucleoside PhosphatesEscherichia coliGene ExpressionKineticsListeriaMultigene FamilyNudix HydrolasesPyrophosphatasesRecombinant Proteins8-oxodeoxyadenosine triphosphateAdenosine Diphosphate RiboseBacterial ProteinsDeoxyadenine NucleotidesDeoxyguanine Nucleotidesdeoxyguanosine triphosphateDinucleoside PhosphatesNudix HydrolasesPyrophosphatasesRecombinant ProteinskineticsNudixphysiological substratesubstrate screening

Identifiers

PMID27618147
PMCPMC5158307

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.