ArticleInternational journal for parasitology. Parasites and wildlife2016
Molecular characterization of trypanosomatid infections in wild howler monkeys (Alouatta caraya) in northeastern Argentina.
Article in International journal for parasitology. Parasites and wildlife, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 28 citations in OpenAlex.
- High infection prevalences by Trypanosoma minasense in non-human primates from the Southeast region of Brazil.Scientific reports · 2024Article
- Redefining infections with trypanosomatids in Neotropical primates: Case study of the white-footed tamarin (International journal for parasitology. Parasites and wildlife · 2024Article
- Entomological inferences highlight the risk of Leishmania transmission in the urban area of Porto Velho, Rondônia, Brazil.PloS one · 2024Article
- Molecular detection ofFrontiers in veterinary science · 2024Article
- First Molecular Identification of Trypanosomes and Absence ofPathogens (Basel, Switzerland) · 2022Article
- Integrated approaches to howler monkey (Frontiers in veterinary science · 2022Article
- Parasites and Other Infectious Agents in Non-human Primates of Argentina.Current tropical medicine reports · 2022Review
- Zoonotic Blood-Borne Pathogens in Non-Human Primates in the Neotropical Region: A Systematic Review.Pathogens (Basel, Switzerland) · 2021Review
- Trypanosomatid Infections among Vertebrates of Chile: A Systematic Review.Pathogens (Basel, Switzerland) · 2020Review
- Article
- Development and evaluation of a duplex TaqMan qPCR assay for detection and quantification of Trypanosoma cruzi infection in domestic and sylvatic reservoir hosts.Parasites & vectors · 2019Article
- UncoveringInternational journal for parasitology. Parasites and wildlife · 2019Article
- Helminth parasites of howler and spider monkeys in Mexico: Insights into molecular diagnostic methods and their importance for zoonotic diseases and host conservation.International journal for parasitology. Parasites and wildlife · 2017Article
- High Trypanosoma spp. diversity is maintained by bats and triatomines in Espírito Santo state, Brazil.PloS one · 2017Article
Corrections and comments
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Authors and funding
4 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The transmission of Trypanosoma cruzi by vectors is confined to the Americas, and the infection circulates in at least two broadly defined transmission cycles occurring in domestic and sylvatic habitats. This study sought to detect and characterize infection by T. cruzi and other trypanosomes using PCR strategies in blood samples from free-ranging howler monkeys, Alouatta caraya, in the northeastern Argentina. Blood samples were collected at four sites with variable levels of habitat modification by human activity. PCR was conducted using primers for kinetoplast DNA, satellite DNA and ribosomal DNA of the trypanosomatid parasites. Ribosomal and satellite DNA fragments were sequenced to identify the trypanosomatid species and to characterize the discrete typing units (DTUs) of T. cruzi. Overall, 46% (50/109) of the howlers were positive according to the kDNA-PCR assay, but only 7 of the howlers were positive according to the SatDNA-PCR protocol. We sequenced the amplicons of the satellite DNA obtained from five specimens, and the sequences were 99% and 100% similar to T. cruzi. A sequence typical of DTU T. cruzi I was found in one howler monkey from the "remote" site, while sequences compatible with DTUs II, V, and VI were found in howlers from the "remote", "rural" and "village" sites. We detected 96% positive samples for RibDNA-PCR, 9 of which were sequenced and displayed 99% identity with Trypanosoma minasense, while none showed identity with T. cruzi. The results demonstrated the presence of T. cruzi and a species closely related to T. minasense in blood samples from free-ranging A. caraya, belonging to different T. cruzi DTUs circulating in these howler monkey populations. The results obtained in this study could help evaluate the role of A. caraya as a reservoir of T. cruzi in regions where Chagas disease is hyper-endemic and where the human-wildlife interface is increasing.
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