ArticleFrontiers in microbiology2016
Whole Genome Sequencing of Enterovirus species C Isolates by High-Throughput Sequencing: Development of Generic Primers.
Article in Frontiers in microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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Who cites it
18 citing papers in PubMed, 29 citations in OpenAlex.
- Phylogenetic analysis of enteroviruses from non-human primates suggests two new species within the genusVirus evolution · 2026Article
- Molecular characterization of enteroviruses in patients with acute respiratory infection and influenza-like illness in the Central African Republic revealed mainly polioviruses and other enteric enterovirus types.Access microbiology · 2026Article
- Prevalence and epidemiology of enterovirus species in a pediatric oncology patient population.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2025Article
- Direct amplification and sequencing of enterovirus genomes from clinical and wastewater samples reveals uncommon virus types in Cameroon.Archives of virology · 2025Article
- Genome characterisation of the first isolate of human enterovirus c99 from an acute flaccid paralysis case in Brazil.Memorias do Instituto Oswaldo Cruz · 2025Article
- Article
- Opening a 60-year time capsule: sequences of historical poliovirus cold variants shed a new light on a contemporary strain.Virus evolution · 2024Article
- Laboratory diagnosis of nonpolio enteroviruses: A review of the current literature.Biosafety and health · 2023Review
- Detection of recombinant breakpoint in the genome of human enterovirus E11 strain associated with a fatal nosocomial outbreak.Virology journal · 2022Article
- Development of a New Internally Controlled One-Step Real-Time RT-PCR for the Molecular Detection of Enterovirus A71 in Africa and Madagascar.Frontiers in microbiology · 2020Article
- Genetic and phenotypic characterization of recently discovered enterovirus D type 111.PLoS neglected tropical diseases · 2019Article
- Reference Echovirus 7 and 19 Genomes from Nigeria.Microbiology resource announcements · 2018Article
- Extending the utility of the WHO recommended assay for direct detection of enteroviruses from clinical specimen for resolving poliovirus co-infection.BMC research notes · 2018Article
- Detection by Direct Next Generation Sequencing Analysis of Emerging Enterovirus D68 and C109 Strains in an Environmental Sample From Scotland.Frontiers in microbiology · 2018Article
- Whole Genome Sequencing of Enteroviruses Species A to D by High-Throughput Sequencing: Application for Viral Mixtures.Frontiers in microbiology · 2018Article
- Study the Features of 57 Confirmed CRISPR Loci in 38 Strains ofFrontiers in microbiology · 2018Article
- Revealing enterovirus infection in chronic human disorders: An integrated diagnostic approach.Scientific reports · 2017Article
- Comparison of Algorithms for the Detection of Enteroviruses in Stool Specimens from Children Diagnosed with Acute Flaccid Paralysis.Journal of pathogens · 2017Article
Corrections and comments
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Authors and funding
10 authors at 5 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Enteroviruses are among the most common viruses infecting humans and can cause diverse clinical syndromes ranging from minor febrile illness to severe and potentially fatal diseases. Enterovirus species C (EV-C) consists of more than 20 types, among which the three serotypes of polioviruses, the etiological agents of poliomyelitis, are included. Biodiversity and evolution of EV-C genomes are shaped by frequent recombination events. Therefore, identification and characterization of circulating EV-C strains require the sequencing of different genomic regions. A simple method was developed to quickly sequence the entire genome of EV-C isolates. Four overlapping fragments were produced separately by RT-PCR performed with generic primers. The four amplicons were then pooled and purified prior to being sequenced by a high-throughput technique. The method was assessed on a panel of EV-Cs belonging to a wide-range of types. It can be used to determine full-length genome sequences through de novo assembly of thousands of reads. It was also able to discriminate reads from closely related viruses in mixtures. By decreasing the workload compared to classical Sanger-based techniques, this method will serve as a precious tool for sequencing large panels of EV-Cs isolated in cell cultures during environmental surveillance or from patients, including vaccine-derived polioviruses.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.