ArticleMolecular and cellular biology1989
Promoter upstream elements of the chicken cardiac myosin light-chain 2-A gene interact with trans-acting regulatory factors for muscle-specific transcription.
Article in Molecular and cellular biology, 1989. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 77 citations in OpenAlex.
- Actin-related protein 5 suppresses the cooperative activation of cardiac gene transcription by myocardin and MEF2.FEBS open bio · 2023Article
- Molecular and functional analyses of the fast skeletal myosin light chain2 gene of the Korean oily bitterling, Acheilognathus koreensis.International journal of molecular sciences · 2013Article
- ERK5 is a novel type of mitogen-activated protein kinase containing a transcriptional activation domain.Molecular and cellular biology · 2000Article
- A network of mitogen-activated protein kinases links G protein-coupled receptors to the c-jun promoter: a role for c-Jun NH2-terminal kinase, p38s, and extracellular signal-regulated kinase 5.Molecular and cellular biology · 1999Article
- Combinatorial control of muscle development by basic helix-loop-helix and MADS-box transcription factors.Proceedings of the National Academy of Sciences of the United States of America · 1996Review
- Distinct regulatory elements control muscle-specific, fiber-type-selective, and axially graded expression of a myosin light-chain gene in transgenic mice.Molecular and cellular biology · 1996Article
- Mutational analysis of the DNA binding, dimerization, and transcriptional activation domains of MEF2C.Molecular and cellular biology · 1996Article
- Activation of a muscle-specific enhancer by the Ski proto-oncogene.Nucleic acids research · 1995Article
- Regulatory role of MEF2D in serum induction of the c-jun promoter.Molecular and cellular biology · 1995Article
- Identification of positive and negative transcriptional regulatory elements of the rabbit angiotensin-converting enzyme gene.Nucleic acids research · 1994Article
- A novel myogenic regulatory circuit controls slow/cardiac troponin C gene transcription in skeletal muscle.Molecular and cellular biology · 1994Article
- A Mef2 gene that generates a muscle-specific isoform via alternative mRNA splicing.Molecular and cellular biology · 1994Article
- cis-acting sequences of the rat troponin I slow gene confer tissue- and development-specific transcription in cultured muscle cells as well as fiber type specificity in transgenic mice.Molecular and cellular biology · 1993Article
- Regulation of the human cardiac/slow-twitch troponin C gene by multiple, cooperative, cell-type-specific, and MyoD-responsive elements.Molecular and cellular biology · 1993Article
- Myocyte enhancer factor (MEF) 2C: a tissue-restricted member of the MEF-2 family of transcription factors.Proceedings of the National Academy of Sciences of the United States of America · 1993Article
- Multiple regulatory elements contribute differentially to muscle creatine kinase enhancer activity in skeletal and cardiac muscle.Molecular and cellular biology · 1993Article
- hMEF2C gene encodes skeletal muscle- and brain-specific transcription factors.Molecular and cellular biology · 1993Article
- Gtx: a novel murine homeobox-containing gene, expressed specifically in glial cells of the brain and germ cells of testis, has a transcriptional repressor activity in vitro for a serum-inducible promoter.The EMBO journal · 1993Article
- A new serum-responsive, cardiac tissue-specific transcription factor that recognizes the MEF-2 site in the myosin light chain-2 promoter.Molecular and cellular biology · 1993Article
- Tissue-specific restriction of skeletal muscle troponin C gene expression.Gene expression · 1993Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A segment of the 5'-flanking region of the chicken cardiac myosin light-chain gene extending from nucleotide -64 to the RNA start site is sufficient to allow muscle-specific transcription. In this paper, we characterize, by mutational analysis, sequence elements which are essential for the promoter activity. Furthermore, we present evidence for a negative-acting element which is possibly involved in conferring the muscle specificity. Nuclear proteins specifically bind to the DNA elements, as demonstrated by gel mobility shift assays and DNase I protection footprinting. The significance of the DNA-protein interactions for the function of the promoter in vivo is demonstrated by competition experiments in which protein-binding oligonucleotides were microinjected into nuclei of myotubes, where they successfully competed for the protein factors which are required to trans activate the MLC2-A promoter. The ability to bind nuclear proteins involves two closely spaced AT-rich sequence elements, one of which constitutes the TATA box. The binding properties correlate well with the capacity to activate transcription in vivo, since mutations in this region of the promoter concomitantly lead to loss of binding and transcriptional activity.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.