ArticleScientific reports2016
Effect of X-ray irradiation on ancient DNA in sub-fossil bones - Guidelines for safe X-ray imaging.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 97 citations in OpenAlex.
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- An infant burial from Arma Veirana in northwestern Italy provides insights into funerary practices and female personhood in early Mesolithic Europe.Scientific reports · 2021Article
- A robust, semi-automated approach for counting cementum increments imaged with synchrotron X-ray computed tomography.PloS one · 2021Article
- Cardiac multi-scale investigation of the right and left ventricleCardiovascular diagnosis and therapy · 2020Review
- Ancient dental pulp: Masterpiece tissue for paleomicrobiology.Molecular genetics & genomic medicine · 2020Review
- Building three-dimensional models before destructive sampling of bioarchaeological remains: a comment to PálsdóttirRoyal Society open science · 2020Article
- X-ray computed tomography in life sciences.BMC biology · 2020Review
- The utility of micro-computed tomography for the non-destructive study of eye microstructure in snails.Scientific reports · 2019Article
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Corrections and comments
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Authors and funding
20 authors at 9 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sub-fossilised remains may still contain highly degraded ancient DNA (aDNA) useful for palaeogenetic investigations. Whether X-ray computed [micro-] tomography ([μ]CT) imaging of these fossils may further damage aDNA remains debated. Although the effect of X-ray on DNA in living organisms is well documented, its impact on aDNA molecules is unexplored. Here we investigate the effects of synchrotron X-ray irradiation on aDNA from Pleistocene bones. A clear correlation appears between decreasing aDNA quantities and accumulating X-ray dose-levels above 2000 Gray (Gy). We further find that strong X-ray irradiation reduces the amount of nucleotide misincorporations at the aDNA molecule ends. No representative effect can be detected for doses below 200 Gy. Dosimetry shows that conventional μCT usually does not reach the risky dose level, while classical synchrotron imaging can degrade aDNA significantly. Optimised synchrotron protocols and simple rules introduced here are sufficient to ensure that fossils can be scanned without impairing future aDNA studies.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.