ArticleJournal of ovarian research2016
Expression pattern of circadian genes and steroidogenesis-related genes after testosterone stimulation in the human ovary.
Article in Journal of ovarian research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 50 citations in OpenAlex.
- The Circadian Rhythm Regulates the Hepato-ovarian Axis Linking Polycystic Ovary Syndrome and Non-alcoholic Fatty Liver Disease.Biochemical genetics · 2026Article
- Ovarian Rev-erbα: a central regulator of fertility following chronodisruption.Frontiers in endocrinology · 2026Article
- Circadian rhythm disruption and polycystic ovary syndrome: a systematic review and meta-analysis.AJOG global reports · 2025Review
- Sleep traits and risk of premature ovarian insufficiency - two-sample and multivariate mendelian randomisation analysis.Przeglad menopauzalny = Menopause review · 2025Article
- Twelve-hour ultradian rhythmic reprogramming of gene expression in the human ovary during aging.Journal of assisted reproduction and genetics · 2025Article
- Aquaporin 2 is differentially expressed in granulosa cells of various stages of human follicles and is regulated by luteinizing hormone.Frontiers in cell and developmental biology · 2025Article
- Getting Up in the Morning and Chronotype in Relation to Polycystic Ovarian Syndrome: A Mendelian Randomization and Cross-Sectional Study.Nature and science of sleep · 2025Article
- Period circadian regulator 2-mediated steroid hormone synthesis by regulating transcription of steroidogenic acute regulatory protein in porcine granulosa cells.Journal of animal science · 2024Article
- CLOCK inhibits the proliferation of porcine ovarian granulosa cells by targeting ASB9.Journal of animal science and biotechnology · 2023Article
- Mutual Shaping of Circadian Body-Wide Synchronization by the Suprachiasmatic Nucleus and Circulating Steroids.Frontiers in behavioral neuroscience · 2022Review
- Ovarian activation delays in peripubertal ewe lambs infected with Haemonchus contortus can be avoided by supplementing protein in their diets.BMC veterinary research · 2021Article
- Circadian Rhythms Within the Female HPG Axis: From Physiology to Etiology.Endocrinology · 2021Review
- Circadian Clock Genes REV-ERBs Inhibits Granulosa Cells Apoptosis by Regulating Mitochondrial Biogenesis and Autophagy in Polycystic Ovary Syndrome.Frontiers in cell and developmental biology · 2021Article
- Disruption of Circadian Rhythms: A Crucial Factor in the Etiology of Infertility.International journal of molecular sciences · 2020Review
- Is the aging human ovary still ticking?: Expression of clock-genes in luteinized granulosa cells of young and older women.Journal of ovarian research · 2018Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundPrevious studies have shown that circadian genes might be involved in the development of polycystic ovarian syndrome (PCOS). Hyperandrogenism is a hallmark feature of PCOS. However, the effect of hyperandrogenism on circadian gene expression in human granulosa cells is unknown, and the general expression pattern of circadian genes in the human ovary is unclear.
methodsExpression of the circadian proteins CLOCK and PER2 in human ovaries was observed by immunohistochemistry. The mRNA expression patterns of the circadian genes CLOCK, PER2, and BMAL1, and the steroidogenesis-related genes STAR, CYP11A1, HSD3B2, and CYP19A1 in cultured human luteinized granulosa cells were analyzed over the course of 48 h after testosterone treatment by quantitative polymerase chain reaction.
resultsImmunostaining of CLOCK and PER2 protein was detected in the granulosa cells of dominant antral follicles but was absent in the primordial, primary, or preantral follicles of human ovaries. After testosterone stimulation, expression of PER2 showed an oscillating pattern, with two peaks occurring at the 24th and 44th hours; expression of CLOCK increased significantly to the peak at the 24th hour, whereas expression of BMAL1 did not change significantly over time in human luteinized granulosa cells. Among the four steroidogenesis-related genes evaluated, only STAR displayed an oscillating expression pattern with two peaks occurring at the 24th and 40th hours after testosterone stimulation.
conclusionsCircadian genes are expressed in the dominant antral follicles of the human ovary. Oscillating expression of the circadian gene PER2 can be induced by testosterone in human granulosa cells in vitro. Expression of STAR also displayed an oscillating pattern after testosterone stimulation. Our results indicate a potential relationship between the circadian clock and steroidogenesis in the human ovary, and demonstrate the effect of testosterone on circadian gene expression in granulosa cells.
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