ArticleJournal of assisted reproduction and genetics2016
Protein expression in human cumulus cells as an indicator of blastocyst formation and pregnancy success.
Article in Journal of assisted reproduction and genetics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
8 citing papers in PubMed.
- Maturational competence of equine oocytes is associated with alterations in their 'cumulome'.Molecular human reproduction · 2024Article
- Nonerythroid hemoglobin promotes human cumulus cell viability and the developmental capacity of the human oocyte.F&S science · 2023Article
- Implementing a preimplantation proteomic approach to advance assisted reproduction technologies in the framework of predictive, preventive, and personalized medicine.The EPMA journal · 2022Review
- Extracellular cell-free RNA profile in human large follicles and small follicles.Frontiers in cell and developmental biology · 2022Article
- The Human Ovary and Future of Fertility Assessment in the Post-Genome Era.International journal of molecular sciences · 2019Review
- Analyzing the Transcriptome Profile of Human Cumulus Cells Related to Embryo Quality via RNA Sequencing.BioMed research international · 2018Article
- Biobanking of different body fluids within the frame of IVF-a standard operating procedure to improve reproductive biology research.Journal of assisted reproduction and genetics · 2017Article
- The Use of Proteomics in Assisted Reproduction.In vivo (Athens, Greece)Review
Corrections and comments
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeThe goal for the present study was to implement a technique for protein extraction and identification in human cumulus cells (CCs).
methodsForty samples of CCs were collected after ovum pick-up from patients undergoing intracytoplasmic sperm injection (ICSI). Samples were split into the blastocyst group (n = 10), including patients in which all embryos converted into blastocysts, and the non-blastocyst group (n = 10), including patients in which none of the embryos reached the blastocyst stage or the positive-pregnancy (n = 10) and negative-pregnancy group (n = 10). Proteins were extracted and injected into a liquid chromatography system coupled to a mass spectrometer. The spectra were processed and used to search a database.
resultsThere were 87 different proteins in samples from the blastocyst and non-blastocyst groups, in which 30 were exclusively expressed in the blastocyst group and 17 in the non-blastocyst group. Among the 72 proteins detected in the pregnancy groups, 19 were exclusively expressed in the positive, and 16 were exclusively expressed in the negative-pregnancy group.
conclusionsCC proteomics may be useful for predicting pregnancy success and the identification of patients that should be included in extended embryo culture programs.
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Registered trials
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