ArticleStem cell research & therapy2016
Two-photon polymerized "nichoid" substrates maintain function of pluripotent stem cells when expanded under feeder-free conditions.
Article in Stem cell research & therapy, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Nanoengineered 3D culture substrate enables superior persistence and polyclonal engraftment of genetically engineered hematopoietic stem cells.Cell stem cell · 2026Article
- A Tridimensional in vitro Epithelial Model of a Simulated Vaginal Environment for the Study ofIntervirology · 2026Article
- Article
- 3D photopolymerized microstructured scaffolds influence nuclear deformation, nucleo/cytoskeletal protein organization, and gene regulation in mesenchymal stem cells.APL bioengineering · 2023Article
- Two-Photon Polymerization: Fundamentals, Materials, and Chemical Modification Strategies.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Review
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- Article
- Whole transcriptomic analysis of mesenchymal stem cells cultured in Nichoid micro-scaffolds.Frontiers in bioengineering and biotechnology · 2022Article
- Geometrically defined environments direct cell division rate and subcellular YAP localization in single mouse embryonic stem cells.Scientific reports · 2021Article
- Neural precursors cells expanded in a 3D micro-engineered niche present enhanced therapeutic efficacyNanotheranostics · 2021Article
- Characterization of the Microflow Through 3D Synthetic Niche Microenvironments Hosted in a Millifluidic Bioreactor.Frontiers in bioengineering and biotechnology · 2021Article
- Characterization of Mesenchymal Stem Cell Differentiation within Miniaturized 3D Scaffolds through Advanced Microscopy Techniques.International journal of molecular sciences · 2020Article
- Dissecting the Effect of a 3D Microscaffold on the Transcriptome of Neural Stem Cells with Computational Approaches: A Focus on Mechanotransduction.International journal of molecular sciences · 2020Article
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- Review
- Tissue engineering and regenerative medicine strategies for the female breast.Journal of tissue engineering and regenerative medicine · 2020Review
- Bioengineering tools to speed up the discovery and preclinical testing of vaccines for SARS-CoV-2 and therapeutic agents for COVID-19.Theranostics · 2020Review
- Multi-foci laser microfabrication of 3D polymeric scaffolds for stem cell expansion in regenerative medicine.Scientific reports · 2019Article
- Three-dimensional system enabling the maintenance and directed differentiation of pluripotent stem cells under defined conditions.Science advances · 2017Article
- Scaling-Up Techniques for the Nanofabrication of Cell Culture Substrates via Two-Photon Polymerization for Industrial-Scale Expansion of Stem Cells.Materials (Basel, Switzerland) · 2017Article
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe use of pluripotent cells in stem cell therapy has major limitations, mainly related to the high costs and risks of exogenous conditioning and the use of feeder layers during cell expansion passages.
methodsWe developed an innovative three-dimensional culture substrate made of "nichoid" microstructures, nanoengineered via two-photon laser polymerization. The nichoids limit the dimension of the adhering embryoid bodies during expansion, by counteracting cell migration between adjacent units of the substrate by its microarchitecture. We expanded mouse embryonic stem cells on the nichoid for 2 weeks. We compared the expression of pluripotency and differentiation markers induced in cells with that induced by flat substrates and by a culture layer made of kidney-derived extracellular matrix.
resultsThe nichoid was found to be the only substrate, among those tested, that maintained the expression of the OCT4 pluripotency marker switched on and, simultaneously, the expression of the differentiation markers GATA4 and α-SMA switched off. The nichoid promotes pluripotency maintenance of embryonic stem cells during expansion, in the absence of a feeder layer and exogenous conditioning factors, such as the leukocyte inhibitory factor.
conclusionsWe hypothesized that the nichoid microstructures induce a genetic reprogramming of cells by controlling their cytoskeletal tension. Further studies are necessary to understand the exact mechanism by which the physical constraint provided by the nichoid architecture is responsible for cell reprogramming. The nichoid may help elucidate mechanisms of pluripotency maintenance, while potentially cutting the costs and risks of both feed-conditioning and exogenous conditioning for industrial-scale expansion of stem cells.
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