ArticleParasites & vectors2016
Use of MALDI-TOF MS and culturomics to identify mosquitoes and their midgut microbiota.
Article in Parasites & vectors, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers, 1 of them a synthesis that pooled it.
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Who cites it
29 citing papers in PubMed, 1 synthesis or guideline pooled it.
- MALDI-TOF mass spectrometry as a diagnostic tool in human and veterinary helminthology: a systematic review.Parasites & vectors · 2019Pooled it
- Microbiota variation inMicrobiology spectrum · 2026Article
- Mosquitoes as vector-based sentinels for tracking bacterial pathogens and antimicrobial resistance in the environment.Frontiers in microbiology · 2026Review
- Ecological and engineered modulation of the mosquito microbiome: mechanisms, vector competence, and translational prospects for disease control.Frontiers in microbiology · 2026Review
- Diversity of Gut Bacteria of Field-CollectedInsects · 2025Article
- MALDI-TOF MS Profiling and Its Contribution to Mosquito-Borne Diseases: A Systematic Review.Insects · 2024Review
- MALDI-TOF MS Is an Effective Technique To Classify Specific Microbiota.Microbiology spectrum · 2023Article
- Isolation and identification of microbiota ofIranian journal of microbiology · 2023Article
- Anopheline mosquito saliva contains bacteria that are transferred to a mammalian host through blood feeding.Frontiers in microbiology · 2023Article
- Enhanced procedures for mosquito identification by MALDI-TOF MS.Parasites & vectors · 2022Article
- Use of the proteomic tool MALDI-TOF MS in termite identification.Scientific reports · 2022Article
- Microbiota identified from preserved Anopheles.Malaria journal · 2021Article
- Identification of mixed and successive blood meals of mosquitoes using MALDI-TOF MS protein profiling.Parasitology · 2020Article
- Metagenomic analysis of Aedes aegypti and Culex quinquefasciatus mosquitoes from Grenada, West Indies.PloS one · 2020Article
- Bacterial Microbiome in Wild-CaughtFrontiers in microbiology · 2020Article
- Identification of French Guiana anopheline mosquitoes by MALDI-TOF MS profiling using protein signatures from two body parts.PloS one · 2020Article
- Microbiota potentialized larvicidal action of imidazolium salts against Aedes aegypti (Diptera: Culicidae).Scientific reports · 2019Article
- The contribution of gut bacteria to insecticide resistance and the life histories of the major malaria vector Anopheles arabiensis (Diptera: Culicidae).Scientific reports · 2019Article
- Isolation and identification of microflora from the midgut and salivary glands of Anopheles species in malaria endemic areas of Ethiopia.BMC microbiology · 2019Article
- Application of matrix-assisted laser desorption/ionization mass spectrometry to identify species of Neotropical Anopheles vectors of malaria.Malaria journal · 2019Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMosquitoes transmit a wide range of human parasitic and viral diseases. In recent years, new techniques such as MALDI-TOF MS have been developed to identify mosquitoes at the species level, which is key for entomological surveys. Additionally, there is increasing interest in the mosquito microbiota and its role in vector capacity.
methodsThe culturomics approach previously used in our laboratory to study human gut microbiota was applied to evaluate the midgut bacterial diversity of Anopheles gambiae (wild and laboratory strains), Aedes albopictus (wild and laboratory strains) and Culex quinquefasciatus (wild strains) in order to determine the influence of the environmental status on the midgut microbiota of the mosquitoes.
resultsMosquitoes collected in the field were accurately identified by MALDI-TOF MS analysis of their legs. Adult mosquito midgut microbiota was composed of four phyla, including Proteobacteria, Bacteroidetes, Actinobacteria and Firmicutes. The majority of the bacteria detected in the microbiota of mosquitoes were gram-negative and belong to the phylum Proteobacteria. MALDI-TOF MS identified for the first time a new bacterial species from An. gambiae midgut microbiota.
conclusionIn this study, the culturomics approach was found to be a reliable technique for exploring the diversity of the mosquito microbiota. MALDI-TOF MS was confirmed as a promising technique to identify mosquitoes collected in the field. Culturomics allowed the isolation of a new bacterial species not previously associated with mosquito vectors. The environment plays a role in the bacterial diversity of the microbiota, which could enable the development of new control strategies for mosquito-borne disease.
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