Evidence map›Paper›PMID 27612810›Full record

ArticleMaterials science & engineering. C, Materials for biological applications2016

Injectable calcium phosphate with hydrogel fibers encapsulating induced pluripotent, dental pulp and bone marrow stem cells for bone repair.

Lin Wang, Chi Zhang, Chunyan Li, Michael D Weir, Ping Wang, Mark A Reynolds, Liang Zhao, Hockin H K Xu

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Materials science & engineering. C, Materials for biological applications, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.

0numbers the graph read from it
0cells of the map it votes in
29citing papers in PubMed
3.8field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

29 citing papers in PubMed, 63 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 4 institutions in 2 countries.

Lin WangVIP Integrated Department, School and Hospital of Stomatology, Jilin University, Changchun, Jilin 130011,China; Department of Endodontics, Periodontics and Prosthodontics, University of Maryland School of Dentistry, Baltimore, MD 21201, USA.
Chi ZhangDepartment of Endodontics, Periodontics and Prosthodontics, University of Maryland School of Dentistry, Baltimore, MD 21201, USA; State Key Laboratory of Oral Diseases, West China School of Stomatology, Sichuan University, Chengdu 610041, China.
Chunyan LiVIP Integrated Department, School and Hospital of Stomatology, Jilin University, Changchun, Jilin 130011,China.
Michael D WeirDepartment of Endodontics, Periodontics and Prosthodontics, University of Maryland School of Dentistry, Baltimore, MD 21201, USA.
Ping WangDepartment of Endodontics, Periodontics and Prosthodontics, University of Maryland School of Dentistry, Baltimore, MD 21201, USA. Electronic address: pwang@umaryland.edu.
Mark A ReynoldsDepartment of Endodontics, Periodontics and Prosthodontics, University of Maryland School of Dentistry, Baltimore, MD 21201, USA.
Liang ZhaoDepartment of Endodontics, Periodontics and Prosthodontics, University of Maryland School of Dentistry, Baltimore, MD 21201, USA; Department of Orthopaedic Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, China. Electronic address: lzhaonf@126.com.
Hockin H K XuDepartment of Endodontics, Periodontics and Prosthodontics, University of Maryland School of Dentistry, Baltimore, MD 21201, USA; Center for Stem Cell Biology & Regenerative Medicine, University of Maryland School of Medicine, Baltimore, MD 21201, USA; Department of Mechanical Engineering, University of Maryland Baltimore County, Baltimore County, MD 21250, USA.
University of Maryland, Baltimore · USJilin University · CNNanfang Hospital · CNSichuan University · CN

Funding

Injectable and Strong Nano-Apatite/Stem Cell Scaffolds for Bone RegenerationR01DE014190 · NIDCR · UNIVERSITY OF MARYLAND BALTIMORE · PI XU, HUAKUN · 2003 to 2013
$3.4M
Human Embryonic Stem Cells in Calcium Phosphate Constructs for Bone RegenerationR21DE022625 · NIDCR · UNIVERSITY OF MARYLAND BALTIMORE · PI XU, HUAKUN · 2012 to 2013
$413k
NIDCR NIH HHS R01 DE014190NIDCR NIH HHS R21 DE022625
6 · The paper itself

Abstract

Human induced pluripotent stem cell-derived mesenchymal stem cells (hiPSC-MSCs), dental pulp stem cells (hDPSCs) and bone marrow MSCs (hBMSCs) are exciting cell sources in regenerative medicine. However, there has been no report comparing hDPSCs, hBMSCs and hiPSC-MSCs for bone engineering in an injectable calcium phosphate cement (CPC) scaffold. The objectives of this study were to: (1) develop a novel injectable CPC containing hydrogel fibers encapsulating stem cells for bone engineering, and (2) compare cell viability, proliferation and osteogenic differentiation of hDPSCs, hiPSC-MSCs from bone marrow (BM-hiPSC-MSCs) and from foreskin (FS-hiPSC-MSCs), and hBMSCs in CPC for the first time. The results showed that the injection did not harm cell viability. The porosity of injectable CPC was 62%. All four types of cells proliferated and differentiated down the osteogenic lineage inside hydrogel fibers in CPC. hDPSCs, BM-hiPSC-MSCs, and hBMSCs exhibited high alkaline phosphatase, runt-related transcription factor, collagen I, and osteocalcin gene expressions. Cell-synthesized minerals increased with time (p<0.05), with no significant difference among hDPSCs, BM-hiPSC-MSCs and hBMSCs (p>0.1). Mineralization by hDPSCs, BM-hiPSC-MSCs, and hBMSCs inside CPC at 14d was 14-fold that at 1d. FS-hiPSC-MSCs were inferior in osteogenic differentiation compared to the other cells. In conclusion, hDPSCs, BM-hiPSC-MSCs and hBMSCs are similarly and highly promising for bone tissue engineering; however, FS-hiPSC-MSCs were relatively inferior in osteogenesis. The novel injectable CPC with cell-encapsulating hydrogel fibers may enhance bone regeneration in dental, craniofacial and orthopedic applications.

Indexed as

Tissue EngineeringAlginatesBone Marrow CellsBone RegenerationCalcium PhosphatesCell Culture TechniquesCell DifferentiationCells, CulturedCell SurvivalChitosanCollagen Type ICore Binding FactorsDental PulpFibrinGlucuronic AcidHexuronic AcidsAlginatescalcium phosphateCalcium PhosphatesChitosanCollagen Type ICore Binding FactorsFibrinGlucuronic AcidHexuronic AcidsHydrogel, Polyethylene Glycol DimethacrylateOsteocalcinBone marrow stem cellsCalcium phosphate cementCell-encapsulating fibersDental pulp stem cellsHuman induced pluripotent stem cells

Identifiers

PMID27612810
PMCPMC5609678
OpenAlexW2518457481

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.