ArticleStem cell research & therapy2016
Human fetal mesenchymal stem cell secretome enhances bone consolidation in distraction osteogenesis.
Article in Stem cell research & therapy, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 79 citations in OpenAlex.
- Mechanical‑immune feedback regulation in distraction osteogenesis: From mechanotransduction to immune microenvironment remodeling (Review).Molecular medicine reports · 2026Review
- Effects of mesenchymal stem cell secretome on osteoclast- and osteogenesis-related markers during orthodontic relapse.Journal of Taibah University Medical Sciences · 2026Article
- Propolis nanoemulsion and mesenchymal stem cell conditioned medium promote osteoblastogenesis against lipopolysaccharide-induced osteolysis in hyperglycemic rats.Scientific reports · 2026Article
- Augmenting osteoporotic bone regeneration through a hydrogel-based rejuvenating microenvironment.Bioactive materials · 2024Article
- Diabetes mellitus impairs bone regeneration and biomechanics.Journal of orthopaedic surgery and research · 2023Article
- Article
- High-fat diet causes undesirable bone regeneration by altering the bone marrow environment in rats.Frontiers in endocrinology · 2023Article
- Human fetal mesenchymal stem cells secretome promotes scarless diabetic wound healing through heat-shock protein family.Bioengineering & translational medicine · 2023Article
- Periosteum-inspired in situ CaP generated nanocomposite hydrogels with strong bone adhesion and superior stretchability for accelerated distraction osteogenesis.Biomaterials research · 2022Article
- Article
- Bioactive Scaffold Fabricated by 3D Printing for Enhancing Osteoporotic Bone Regeneration.Bioengineering (Basel, Switzerland) · 2022Article
- Effect and Mechanism of siRNAs Targeting IL-1β/TNF-α Combined with BMSCs Transplantation in Ameliorating Rheumatoid Arthritis in Rats.Veterinary sciences · 2022Article
- PTHrP promotes subchondral bone formation in TMJ-OA.International journal of oral science · 2022Article
- Development of secretome-based strategies to improve cell culture protocols in tissue engineering.Scientific reports · 2022Article
- Therapeutic Potential of Human Fetal Mesenchymal Stem Cells in Musculoskeletal Disorders: A Narrative Review.International journal of molecular sciences · 2022Review
- Implantable Electrical Stimulation at Dorsal Root Ganglions Accelerates Osteoporotic Fracture Healing via Calcitonin Gene-Related Peptide.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Article
- High slew rate pulsed electromagnetic field enhances bone consolidation and shortens daily treatment duration in distraction osteogenesis.Bone & joint research · 2021Article
- Secretome improves testosterone and androgen-binding protein production in testicular dysfunction rats induced by cisplatin.Journal of advanced veterinary and animal research · 2021Article
- Extracellular vesicles in bone and periodontal regeneration: current and potential therapeutic applications.Cell & bioscience · 2021Review
- Conditioned media from endothelial progenitor cells cultured in simulated microgravity promote angiogenesis and bone fracture healing.Stem cell research & therapy · 2021Article
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Authors and funding
10 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundDistraction osteogenesis (DO) is one of the most dramatic reconstructive techniques for inducing bone regeneration, but it involves an undesirably long period for bone consolidation. Developing innovative approaches to enhance bone consolidation is a burning need. Human fetal mesenchymal stem cells (hFMSCs) have been shown to express more primitive developmental genes than those of human adult mesenchymal stem cells (hAMSCs), which is a preferable source for cell therapy and tissue regeneration. In the present study, we investigated the immunogenicity of using the human mesenchymal stem cell (MSC) secretome on rat cells, the effects of secretome on osteogenic differentiation of rat bone marrow-derived MSCs (rBMSCs), and the potential application of hFMSC secretome in promoting bone consolidation in a rat DO model.
methodsSecretome was collected from MSC culture and was used to treat rBMSCs. Following secretome treatment, cell proliferation, alkaline phosphatase staining, Alizarin Red S staining, and mRNA expression of osteogenic differentiation-related genes (including ALP, Runx2, OCN, OPN, and Osx) in the rBMSCs were checked, as well as mixed rat peripheral blood lymphocyte reaction. hFMSC secretome was injected locally into the regenerates from the end of lengthening every 3 days in the rat DO model, until termination. The regenerates were subject to weekly x-rays, micro-computed tomography (μCT) and mechanical testing examination. The bone quality was assessed by histology and immunohistochemistry examinations.
resultsCompared to the secretome from rBMSCs and hAMSCs, hFMSC secretome had the best osteogenic induction ability and low immunogenicity. hFMSC secretome with different doses showed no effect on cell viability. hFMSC secretome at the dose of 100 μg/μl could significantly increase the expression of alkaline phosphatase and all the osteogenic marker genes, as well as the amount of calcium deposits in the rBMSCs. Finally, the local application of hFMSC secretome in distraction regenerates in a rat DO model significantly improved bone consolidation according to the results of μCT, mechanical test, and histological and immunohistochemistry analysis.
conclusionsThe current study demonstrated that hFMSC secretome promotes osteogenesis of rBMSCs and bone consolidation during DO. hFMSC secretome may be a new therapeutic strategy to enhance bone consolidation in patients undergoing DO treatment.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.