ArticlePloS one2016
Detection of African Swine Fever Virus Antibodies in Serum and Oral Fluid Specimens Using a Recombinant Protein 30 (p30) Dual Matrix Indirect ELISA.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
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Who cites it
55 citing papers in PubMed, 103 citations in OpenAlex.
- Development and Evaluation of Three Indirect ELISA Methods Based on Novel Recombinant ASFV Antigens for Serological Detection of African Swine Fever.Pathogens (Basel, Switzerland) · 2026Article
- Sensitivity and Specificity Assessment of Various African Swine Fever ELISA Kits for Accurate Detection of Seropositive Wild Boar.Pathogens (Basel, Switzerland) · 2026Article
- Rapid and visual dual-color lateral flow immunoassay for african swine fever virus antibody detection.Applied microbiology and biotechnology · 2026Article
- Development of a cost-effective serodiagnosis for African swine fever using solubility-enhanced recombinant p54, p30, and p72.Veterinary world · 2025Article
- Oral fluid as a material for serological diagnostics of caseous lymphadenitis in goats.Scientific reports · 2025Article
- Identification of a Conserved Linear Epitope on the p54 Protein of African Swine Fever Virus.Viruses · 2025Article
- Discovery of a Structurally Stable Immunodominant Region in ASFV p30 C-Terminus Using a Panel of Monoclonal Antibodies.Transboundary and emerging diseases · 2025Article
- Pathogenicity and virulence of African swine fever virus.Virulence · 2024Review
- Development and Validation of an Indirect and Blocking ELISA for the Serological Diagnosis of African Swine Fever.Pathogens (Basel, Switzerland) · 2024Article
- Identification of Two Linear Epitopes on MGF_110-13L Protein of African Swine Fever Virus with Monoclonal Antibodies.Animals : an open access journal from MDPI · 2024Article
- Evaluation of early African swine fever virus detection using CP204L gene encoding the p30 protein using quantitative polymerase chain reaction.Veterinary world · 2024Article
- Establishment of an ELISA Based on a Recombinant Antigenic Protein Containing Multiple Prominent Epitopes for Detection of African Swine Fever Virus Antibodies.Microorganisms · 2024Article
- Small ubiquitin-like modifier-tag and modified protein purification significantly increase the quality and quantity of recombinant African swine fever virus p30 protein.Veterinary world · 2024Article
- African Swine Fever Diagnosis in Africa: Challenges and Opportunities.Pathogens (Basel, Switzerland) · 2024Review
- Characterization of the monoclonal antibody and the immunodominant B-cell epitope of African swine fever virus pA104R by using mouse model.Microbiology spectrum · 2024Article
- Article
- A triple protein-based ELISA for differential detection of ASFV antibodies.Frontiers in veterinary science · 2024Article
- African Swine Fever: A Review of Current Disease Management Strategies and Risks Associated with Exhibition Swine in the United States.Animals : an open access journal from MDPI · 2023Review
- Article
- Advanced Strategies for Developing Vaccines and Diagnostic Tools for African Swine Fever.Viruses · 2023Review
Corrections and comments
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Authors and funding
12 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In the absence of effective vaccine(s), control of African swine fever caused by African swine fever virus (ASFV) must be based on early, efficient, cost-effective detection and strict control and elimination strategies. For this purpose, we developed an indirect ELISA capable of detecting ASFV antibodies in either serum or oral fluid specimens. The recombinant protein used in the ELISA was selected by comparing the early serum antibody response of ASFV-infected pigs (NHV-p68 isolate) to three major recombinant polypeptides (p30, p54, p72) using a multiplex fluorescent microbead-based immunoassay (FMIA). Non-hazardous (non-infectious) antibody-positive serum for use as plate positive controls and for the calculation of sample-to-positive (S:P) ratios was produced by inoculating pigs with a replicon particle (RP) vaccine expressing the ASFV p30 gene. The optimized ELISA detected anti-p30 antibodies in serum and/or oral fluid samples from pigs inoculated with ASFV under experimental conditions beginning 8 to 12 days post inoculation. Tests on serum (n = 200) and oral fluid (n = 200) field samples from an ASFV-free population demonstrated that the assay was highly diagnostically specific. The convenience and diagnostic utility of oral fluid sampling combined with the flexibility to test either serum or oral fluid on the same platform suggests that this assay will be highly useful under the conditions for which OIE recommends ASFV antibody surveillance, i.e., in ASFV-endemic areas and for the detection of infections with ASFV isolates of low virulence.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.