ArticleJournal of clinical laboratory analysis2016
An Improved PCR-RFLP Assay for the Detection of a Polymorphism rs2289487 of PLIN1 Gene.
Article in Journal of clinical laboratory analysis, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed, 10 citations in OpenAlex.
- Recent advances and application of whole genome amplification in molecular diagnosis and medicine.MedComm · 2022Review
- Association of DRD2 gene polymorphisms with schizophrenia in the young Bangladeshi population: A pilot study.Heliyon · 2020Article
- An improved PCR-CTPP assay for the detection of ADH1B Arg48His polymorphism.Journal of clinical laboratory analysis · 2018Article
- Detecting the polymorphism of TERF1 gene by an improved PCR-RFLP method.Journal of clinical laboratory analysis · 2018Article
- Application of CRS-PCR-RFLP to identify CYP1A1 gene polymorphism.Journal of clinical laboratory analysis · 2017Article
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundIn recent research, it has been shown that rs2289487 within the PLIN1 gene has different variants that have been associated with obesity, type 2 diabetes, and other diseases. However, the isochizomers such as the BsmI enzyme required for detection of this polymorphism through polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method are expensive. In this study, we aimed to explore a novel PCR-RFLP method for identifying the single-nucleotide polymorphism (SNP) of rs2289487 of PLIN1 gene.
methodsA new restriction enzyme site was created through created restriction site PCR. In the forward primer, a deoxynucleotide A was substituted with C, and after PCR a new restriction enzyme site for BstUI was introduced into the PCR products. A total of 108 samples from Han Chinese were tested to evaluate this new method.
resultsAllele frequencies in the Asian population were 0.326 for allele A and 0.674 for allele G, and the genotype frequencies were 12.8% for AA, 39.5% for AG, and 47.7% for GG.
conclusionThe PCR-RFLP with new site for detecting the SNP of rs2289487 is an improved method with low cost and high accuracy.
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