ArticleXenotransplantation2016
Characterization of immunogenic Neu5Gc in bioprosthetic heart valves.
Article in Xenotransplantation, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 76 citations in OpenAlex.
- Porcine xenotransplantation in the clinical era: converging advances and unresolved barriers on the path to clinical translation - a narrative review.Frontiers in immunology · 2026Review
- Humoral immune responses to hyaluronan oligosaccharides in patients undergoing prosthetic valve surgery.Frontiers in immunology · 2026Article
- Antibody and Cellular Immune Responses in Old α1,3-Galactosyltransferase-Knockout Mice Implanted with Bioprosthetic Heart Valve Tissues.Bioengineering (Basel, Switzerland) · 2025Article
- Discovery and characterization of vertebrate sialoglycan-binding proteins.Seminars in immunology · 2025Review
- Article
- Macrophages as key modulators of calcific aortic valve disease.Frontiers in cardiovascular medicine · 2025Review
- Serum antibody screening using glycan arrays.Chemical Society reviews · 2024Review
- Embedding and Backscattered Scanning Electron Microscopy (EM-BSEM) Is Preferential over Immunophenotyping in Relation to Bioprosthetic Heart Valves.International journal of molecular sciences · 2023Article
- Differential Immune Response to Bioprosthetic Heart Valve Tissues in the α1,3Galactosyltransferase-Knockout Mouse Model.Bioengineering (Basel, Switzerland) · 2023Article
- Oxidative Release ofAnalytical chemistry · 2023Article
- An In Silico Functional Analysis of Non-Synonymous Single-Nucleotide Polymorphisms of BovinePathogens (Basel, Switzerland) · 2023Article
- Immunogenicity of decellularized extracellular matrix scaffolds: a bottleneck in tissue engineering and regenerative medicine.Biomaterials research · 2023Review
- Article
- Oxidative Stress in Structural Valve Deterioration: A Longitudinal Clinical Study.Biomolecules · 2022Article
- Will previous palliative surgery for congenital heart disease be detrimental to subsequent pig heart xenotransplantation?Transplant immunology · 2022Article
- Immune disguise: the mechanisms of Neu5Gc inducing autoimmune and transplant rejection.Genes and immunity · 2022Review
- The role of antibody responses against glycans in bioprosthetic heart valve calcification and deterioration.Nature medicine · 2022Article
- Mechanisms and Drug Therapies of Bioprosthetic Heart Valve Calcification.Frontiers in pharmacology · 2022Review
- The hybrid crosslinking method improved the stability and anti-calcification properties of the bioprosthetic heart valves.Frontiers in bioengineering and biotechnology · 2022Article
- Yak Pericardium as an Alternative Biomaterial for Transcatheter Heart Valves.Frontiers in bioengineering and biotechnology · 2021Article
Corrections and comments
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Authors and funding
15 authors at 9 institutions in 6 countries.
Funding
Abstract
backgroundThe two common sialic acids (Sias) in mammals are N-acetylneuraminic acid (Neu5Ac) and its hydroxylated form N-glycolylneuraminic acid (Neu5Gc). Unlike most mammals, humans cannot synthesize Neu5Gc that is considered foreign and recognized by circulating antibodies. Thus, Neu5Gc is a potential xenogenic carbohydrate antigen in bioprosthetic heart valves (BHV) that tend to deteriorate in time within human patients.
methodsWe investigated Neu5Gc expression in non-engineered animal-derived cardiac tissues and in clinically used commercial BHV, and evaluated Neu5Gc immunogenicity on BHV through recognition by human anti-Neu5Gc IgG.
resultsNeu5Gc was detected by immunohistochemistry in porcine aortic valves and in porcine and bovine pericardium. Qualitative analysis of Sia linkages revealed Siaα2-3>Siaα2-6 on porcine/bovine pericardium while the opposite in porcine aortic/pulmonary valve cusps. Similarly, six commercial BHV containing either porcine aortic valve or porcine/bovine/equine pericardium revealed Siaα2-3>Siaα2-6 expression. Quantitative analysis of Sia by HPLC showed porcine/bovine pericardium express 4-fold higher Neu5Gc levels compared to the porcine aortic/pulmonary valves, with Neu5Ac at 6-fold over Neu5Gc. Likewise, Neu5Gc was expressed on commercial BHV (186.3±16.9 pmol Sia/μg protein), with Neu5Ac at 8-fold over Neu5Gc. Affinity-purified human anti-Neu5Gc IgG showing high specificity toward Neu5Gc-glycans (with no binding to Neu5Ac-glycans) on a glycan microarray, strongly bound to all tested commercial BHV, demonstrating Neu5Gc immune recognition in cardiac xenografts.
conclusionsWe conclusively demonstrated Neu5Gc expression in native cardiac tissues, as well as in six commercial BHV. These Neu5Gc xeno-antigens were recognized by human anti-Neu5Gc IgG, supporting their immunogenicity. Altogether, these findings suggest BHV-Neu5Gc/anti-Neu5Gc may play a role in valve deterioration warranting further investigation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.