ArticleScientific reports2016
Mechanically activated artificial cell by using microfluidics.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- A microfluidic rheometer for tumor mechanics and invasion studies.Lab on a chip · 2025Article
- A microfluidic rheometer for tumor mechanics and invasion studies.bioRxiv : the preprint server for biology · 2025Article
- Viscoelastic recovery times of chondrocytes measured using a novel 3D-printed microfluidic device.Measurement science & technology · 2025Article
- Nanostructured Microsphere Production by Osmotic Extraction of Microfluidic Emulsion Templates.Langmuir : the ACS journal of surfaces and colloids · 2025Article
- Decoding physical principles of cell migration under controlled environment using microfluidics.Biophysics reviews · 2024Review
- Biomedical Applications of Microfluidic Devices: A Review.Biosensors · 2022Review
- Simple One-Step and Rapid Patterning of PDMS Microfluidic Device Wettability for PDMS Shell Production.Frontiers in bioengineering and biotechnology · 2022Article
- Microfluidics for Artificial Life: Techniques for Bottom-Up Synthetic Biology.Micromachines · 2019Review
- PVA-PDMS-Stearic acid composite nanofibrous mats with improved mechanical behavior for selective filtering applications.Scientific reports · 2018Article
- Advanced Microfluidic Device Designed for Cyclic Compression of Single Adherent Cells.Frontiers in bioengineering and biotechnology · 2018Article
- Simultaneous monitoring of transcription and translation in mammalian cell-free expression in bulk and in cell-sized droplets.Synthetic biology (Oxford, England) · 2018Article
- Cell-sized mechanosensitive and biosensing compartment programmed with DNA.Chemical communications (Cambridge, England) · 2017Article
- New advances in probing cell-extracellular matrix interactions.Integrative biology : quantitative biosciences from nano to macro · 2017Review
- Development of an advanced microfluidic micropipette aspiration device for single cell mechanics studies.Biomicrofluidics · 2016Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
All living organisms sense mechanical forces. Engineering mechanosensitive artificial cell through bottom-up in vitro reconstitution offers a way to understand how mixtures of macromolecules assemble and organize into a complex system that responds to forces. We use stable double emulsion droplets (aqueous/oil/aqueous) to prototype mechanosensitive artificial cells. In order to demonstrate mechanosensation in artificial cells, we develop a novel microfluidic device that is capable of trapping double emulsions into designated chambers, followed by compression and aspiration in a parallel manner. The microfluidic device is fabricated using multilayer soft lithography technology, and consists of a control layer and a deformable flow channel. Deflections of the PDMS membrane above the main microfluidic flow channels and trapping chamber array are independently regulated pneumatically by two sets of integrated microfluidic valves. We successfully compress and aspirate the double emulsions, which result in transient increase and permanent decrease in oil thickness, respectively. Finally, we demonstrate the influx of calcium ions as a response of our mechanically activated artificial cell through thinning of oil. The development of a microfluidic device to mechanically activate artificial cells creates new opportunities in force-activated synthetic biology.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.