ArticleBMC cell biology2016
High resolution imaging reveals heterogeneity in chromatin states between cells that is not inherited through cell division.
Article in BMC cell biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
7 citing papers in PubMed.
- Single-chromosome dynamics reveals locus-dependent dynamics and chromosome territory orientation.Journal of cell science · 2023Article
- Studying the Dynamics of Chromatin-Binding Proteins in Mammalian Cells Using Single-Molecule Localization Microscopy.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Molecular characteristics of S-RNase alleles as the determinant of self-incompatibility in the style of Fragaria viridis.Horticulture research · 2021Article
- Hi-D: nanoscale mapping of nuclear dynamics in single living cells.Genome biology · 2020Article
- Chromatin and nucleosome dynamics in DNA damage and repair.Genes & development · 2017Review
- Two loci single particle trajectories analysis: constructing a first passage time statistics of local chromatin exploration.Scientific reports · 2017Article
- The Image Data Resource: A Bioimage Data Integration and Publication Platform.Nature methods · 2017Article
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Authors and funding
7 authors.
Funding
Abstract
backgroundGenomes of eukaryotes exist as chromatin, and it is known that different chromatin states can influence gene regulation. Chromatin is not a static structure, but is known to be dynamic and vary between cells. In order to monitor the organisation of chromatin in live cells we have engineered fluorescent fusion proteins which recognize specific operator sequences to tag pairs of syntenic gene loci. The separation of these loci was then tracked in three dimensions over time using fluorescence microscopy.
resultsWe established a work flow for measuring the distance between two fluorescently tagged, syntenic gene loci with a mean measurement error of 63 nm. In general, physical separation was observed to increase with increasing genomic separations. However, the extent to which chromatin is compressed varies for different genomic regions. No correlation was observed between compaction and the distribution of chromatin markers from genomic datasets or with contacts identified using capture based approaches. Variation in spatial separation was also observed within cells over time and between cells. Differences in the conformation of individual loci can persist for minutes in individual cells. Separation of reporter loci was found to be similar in related and unrelated daughter cell pairs.
conclusionsThe directly observed physical separation of reporter loci in live cells is highly dynamic both over time and from cell to cell. However, consistent differences in separation are observed over some chromosomal regions that do not correlate with factors known to influence chromatin states. We conclude that as yet unidentified parameters influence chromatin configuration. We also find that while heterogeneity in chromatin states can be maintained for minutes between cells, it is not inherited through cell division. This may contribute to cell-to-cell transcriptional heterogeneity.
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