ArticleScientific reports2016
FtsZ does not initiate membrane constriction at the onset of division.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 34 citations in OpenAlex.
- Determining the rate-limiting processes for cell division in Escherichia coli.Nature communications · 2024Article
- Cell constriction requires processive septal peptidoglycan synthase movement independent of FtsZ treadmilling in Staphylococcus aureus.Nature microbiology · 2024Article
- Cryo-EM structure of the bacterial divisome core complex and antibiotic target FtsWIQBL.Nature microbiology · 2023Article
- Using conditional independence tests to elucidate causal links in cell cycle regulation inProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Article
- Coordination mechanism of cell and cyanelle division in the glaucophyte alga Cyanophora sudae.Protoplasma · 2022Article
- Coupling between DNA replication, segregation, and the onset of constriction in Escherichia coli.Cell reports · 2022Article
- Cell-free biogenesis of bacterial division proto-rings that can constrict liposomes.Communications biology · 2020Article
- Bacterial cell division at a glance.Journal of cell science · 2020Review
- pH-dependent activation of cytokinesis modulates Escherichia coli cell size.PLoS genetics · 2020Article
- Roles of the DedD Protein inJournal of bacteriology · 2019Article
- Biological Applications at the Cutting Edge of Cryo-Electron Microscopy.Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada · 2018Review
- Comprehensive analysis of central carbon metabolism illuminates connections between nutrient availability, growth rate, and cell morphology in Escherichia coli.PLoS genetics · 2018Article
- Don't let sleeping dogmas lie: new views of peptidoglycan synthesis and its regulation.Molecular microbiology · 2017Review
- The divisome at 25: the road ahead.Current opinion in microbiology · 2017Review
- Coordination of Chromosome Segregation and Cell Division inFrontiers in microbiology · 2017Article
- The hypermorph FtsA* protein has an in vivo role in relieving the Escherichia coli proto-ring block caused by excess ZapC.PloS one · 2017Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The source of constriction required for division of a bacterial cell remains enigmatic. FtsZ is widely believed to be a key player, because in vitro experiments indicate that it can deform liposomes when membrane tethered. However in vivo evidence for such a role has remained elusive as it has been challenging to distinguish the contribution of FtsZ from that of peptidoglycan-ingrowth. To differentiate between these two possibilities we studied the early stages of division in Escherichia coli, when FtsZ is present at the division site but peptidoglycan synthesizing enzymes such as FtsI and FtsN are not. Our approach was to use correlative cryo-fluorescence and cryo-electron microscopy (cryo-CLEM) to monitor the localization of fluorescently labeled FtsZ, FtsI or FtsN correlated with the septal ultra-structural geometry in the same cell. We noted that the presence of FtsZ at the division septum is not sufficient to deform membranes. This observation suggests that, although FtsZ can provide a constrictive force, the force is not substantial at the onset of division. Conversely, the presence of FtsN always correlated with membrane invagination, indicating that allosteric activation of peptidoglycan ingrowth is the trigger for constriction of the cell envelope during cell division in E. coli.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.