ArticleScientific reports2016
Analyses of point mutation repair and allelic heterogeneity generated by CRISPR/Cas9 and single-stranded DNA oligonucleotides.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Unveiling the cut-and-repair cycle of designer nucleases in human stem and T cells via CLEAR-time dPCR.Nature communications · 2025Article
- Endogenous expression of inactive lysine deacetylases reveals deacetylation-dependent cellular mechanisms.PloS one · 2023Article
- RELA is required for CD271 expression and stem-like characteristics in hypopharyngeal cancer.Scientific reports · 2022Article
- Improving Homology-Directed Repair in Genome Editing Experiments by Influencing the Cell Cycle.International journal of molecular sciences · 2022Review
- Homology directed correction, a new pathway model for point mutation repair catalyzed by CRISPR-Cas.Scientific reports · 2022Article
- Discrimination of single-point mutations in unamplified genomic DNA via Cas9 immobilized on a graphene field-effect transistor.Nature biomedical engineering · 2021Article
- CRISPR/Cas9-mediated correction ofMolecular therapy. Nucleic acids · 2021Article
- A Consensus Model of Homology-Directed Repair Initiated by CRISPR/Cas Activity.International journal of molecular sciences · 2021Review
- Synergistic gene editing in human iPS cells via cell cycle and DNA repair modulation.Nature communications · 2020Article
- Cas9 Mediated Correction ofOncoTargets and therapy · 2020Article
- Interrogating Parkinson's disease associated redox targets: Potential application of CRISPR editing.Free radical biology & medicine · 2019Review
- High fidelity CRISPR/Cas9 increases precise monoallelic and biallelic editing events in primordial germ cells.Scientific reports · 2018Article
- Optimized knock-in of point mutations in zebrafish using CRISPR/Cas9.Nucleic acids research · 2018Article
- Comparative analysis of lipid-mediated CRISPR-Cas9 genome editing techniques.Cell biology international · 2018Article
- Comparative analysis of single-stranded DNA donors to generate conditional null mouse alleles.BMC biology · 2018Article
- Efficient Delivery and Nuclear Uptake Is Not Sufficient to Detect Gene Editing in CD34+ Cells Directed by a Ribonucleoprotein Complex.Molecular therapy. Nucleic acids · 2018Article
- Maternal Supply of Cas9 to Zygotes Facilitates the Efficient Generation of Site-Specific Mutant Mouse Models.PloS one · 2017Article
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6 authors.
Funding
Abstract
The repair of a point mutation can be facilitated by combined activity of a single-stranded oligonucleotide and a CRISPR/Cas9 system. While the mechanism of action of combinatorial gene editing remains to be elucidated, the regulatory circuitry of nucleotide exchange executed by oligonucleotides alone has been largely defined. The presence of the appropriate CRISPR/Cas9 system leads to an enhancement in the frequency of gene editing directed by single-stranded DNA oligonucleotides. While CRISPR/Cas9 executes double-stranded DNA cleavage efficiently, closure of the broken chromosomes is dynamic, as varying degrees of heterogeneity of the cleavage products appear to accompany the emergence of the corrected base pair. We provide a detailed analysis of allelic variance at and surrounding the target site. In one particular case, we report sequence alteration directed by a distinct member of the same gene family. Our data suggests that single-stranded DNA molecules may influence DNA junction heterogeneity created by CRISPR/Cas9.
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