Evidence map›Paper›PMID 27609061›Full record

ArticleThe British journal of nutrition2016

The human milk oligosaccharide 2'-fucosyllactose attenuates the severity of experimental necrotising enterocolitis by enhancing mesenteric perfusion in the neonatal intestine.

Misty Good, Chhinder P Sodhi, Yukihiro Yamaguchi, Hongpeng Jia, Peng Lu, William B Fulton, Laura Y Martin, Thomas Prindle, Diego F Nino, Qinjie Zhou and 5 more

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The British journal of nutrition, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 111 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
111citing papers in PubMed, 1 pooled it
13.4field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

111 citing papers in PubMed, 1 synthesis or guideline pooled it, 175 citations in OpenAlex.

  1. Pooled it
  2. Trial
  3. Trial
  4. Article
  5. Review
  6. Review
  7. Review
  8. Review
  9. Necrotizing enterocolitis.Nature reviews. Disease primers · 2026
    Review
  10. Article
  11. Review
  12. Review
  13. Article
  14. Article
  15. Review
  16. Review
  17. The fifty billion dollar question: does formula cause necrotizing enterocolitis?Journal of perinatology : official journal of the California Perinatal Association · 2025
    Review
  18. Article
  19. An anti-eCIRP strategy for necrotizing enterocolitis.Molecular medicine (Cambridge, Mass.) · 2024
    Article
  20. Article

51 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors at 3 institutions in 1 country.

Misty Good1Divisions of Newborn Medicine,Children's Hospital of Pittsburgh,Pittsburgh,PA 15224,USA.ORCID http://orcid.org/0000-0002-0264-5721
Chhinder P Sodhi3General Pediatric Surgery,Johns Hopkins University and Bloomberg Children's Center,Johns Hopkins Hospital,Baltimore,MD 21287,USA.
Yukihiro Yamaguchi3General Pediatric Surgery,Johns Hopkins University and Bloomberg Children's Center,Johns Hopkins Hospital,Baltimore,MD 21287,USA.
Hongpeng Jia3General Pediatric Surgery,Johns Hopkins University and Bloomberg Children's Center,Johns Hopkins Hospital,Baltimore,MD 21287,USA.
Peng Lu3General Pediatric Surgery,Johns Hopkins University and Bloomberg Children's Center,Johns Hopkins Hospital,Baltimore,MD 21287,USA.
William B Fulton3General Pediatric Surgery,Johns Hopkins University and Bloomberg Children's Center,Johns Hopkins Hospital,Baltimore,MD 21287,USA.
Laura Y Martin3General Pediatric Surgery,Johns Hopkins University and Bloomberg Children's Center,Johns Hopkins Hospital,Baltimore,MD 21287,USA.
Thomas Prindle3General Pediatric Surgery,Johns Hopkins University and Bloomberg Children's Center,Johns Hopkins Hospital,Baltimore,MD 21287,USA.
Diego F Nino3General Pediatric Surgery,Johns Hopkins University and Bloomberg Children's Center,Johns Hopkins Hospital,Baltimore,MD 21287,USA.
Qinjie Zhou3General Pediatric Surgery,Johns Hopkins University and Bloomberg Children's Center,Johns Hopkins Hospital,Baltimore,MD 21287,USA.
Congrong Ma1Divisions of Newborn Medicine,Children's Hospital of Pittsburgh,Pittsburgh,PA 15224,USA.
John A Ozolek5Pediatric Pathology,Children's Hospital of Pittsburgh,Pittsburgh,PA 15224,USA.
Rachael H Buck7Abbott Nutrition,Columbus,OH 43215,USA.
Karen C Goehring7Abbott Nutrition,Columbus,OH 43215,USA.
David J Hackam3General Pediatric Surgery,Johns Hopkins University and Bloomberg Children's Center,Johns Hopkins Hospital,Baltimore,MD 21287,USA.
Bloomberg (United States) · USUniversity of Pittsburgh · USAbbott (United States) · US

Funding

Clinical and Laboratory Research Training for Gastrointestinal SurgeonsT32DK007713 · NIDDK · JOHNS HOPKINS UNIVERSITY · PI DAVID J HACKAM · 1996 to 2026
$5.2M
TLR4 Signaling in the Pathogenesis of Surgical Necrotizing EnterocolitisR01GM078238 · NIGMS · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI HACKAM, DAVID J · 2006 to 2019
$3.9M
Modulation of TLR4 and TLR9 Signaling in NECR01DK083752 · NIDDK · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI HACKAM, DAVID J · 2008 to 2016
$3.4M
Novel Anti-Inflammatory Properties of Breast Milk in Necrotizing EnterocolitisK08DK101608 · NIDDK · WASHINGTON UNIVERSITY · PI GOOD, MISTY L · 2014 to 2017
$584k
NIDDK NIH HHS K08 DK101608NIDDK NIH HHS R01 DK083752NIDDK NIH HHS T32 DK007713NIGMS NIH HHS R01 GM078238
6 · The paper itself

Abstract

Necrotising enterocolitis (NEC) is a common disease in premature infants characterised by intestinal ischaemia and necrosis. The only effective preventative strategy against NEC is the administration of breast milk, although the protective mechanisms remain unknown. We hypothesise that an abundant human milk oligosaccharide (HMO) in breast milk, 2'-fucosyllactose (2'FL), protects against NEC by enhancing intestinal mucosal blood flow, and we sought to determine the mechanisms underlying this protection. Administration of HMO-2'FL protected against NEC in neonatal wild-type mice, resulted in a decrease in pro-inflammatory markers and preserved the small intestinal mucosal architecture. These protective effects occurred via restoration of intestinal perfusion through up-regulation of the vasodilatory molecule endothelial nitric oxide synthase (eNOS), as administration of HMO-2'FL to eNOS-deficient mice or to mice that received eNOS inhibitors did not protect against NEC, and by 16S analysis HMO-2'FL affected the microbiota of the neonatal mouse gut, although these changes do not seem to be the primary mechanism of protection. Induction of eNOS by HMO-2'FL was also observed in cultured endothelial cells, providing a link between eNOS and HMO in the endothelium. These data demonstrate that HMO-2'FL protects against NEC in part through maintaining mesenteric perfusion via increased eNOS expression, and suggest that the 2'FL found in human milk may be mediating some of the protective benefits of breast milk in the clinical setting against NEC.

Indexed as

AnimalsAnimals, NewbornDisease Models, AnimalEnterocolitis, NecrotizingFemaleGene ExpressionHumansInfant, NewbornInfant, Premature, DiseasesIntestinal MucosaMiceMice, Inbred C57BLMice, KnockoutMicrobiotaMilk, HumanNitric Oxide2'-fucosyllactoseNitric OxideNitric Oxide Synthase Type IIITrisaccharides2′FL 2′-fucosyllactose2′-FucosyllactoseBreast milkEndothelial nitric oxide synthaseeNOS endothelial nitric oxide synthaseHMO human milk oligosaccharideHuman milk oligosaccharidesLPS lipopolysaccharideNEC necrotising enterocolitisNecrotising enterocolitisTLR4 toll-like receptor 4

Identifiers

PMID27609061
PMCPMC5124125
OpenAlexW2513325891

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.