Evidence map›Paper›PMID 27608812›Full record

ArticleScientific reports2016

HMGB4 is expressed by neuronal cells and affects the expression of genes involved in neural differentiation.

Ari Rouhiainen, Xiang Zhao, Päivi Vanttola, Kui Qian, Evgeny Kulesskiy, Juha Kuja-Panula, Kathleen Gransalke, Mikaela Grönholm, Emmanual Unni, Marvin Meistrich and 3 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed
0.6field-weighted citation impact, top 30% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed, 23 citations in OpenAlex.

  1. Article
  2. Review
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  4. BiallelicHeliyon · 2024
    Article
  5. Structure and Functions of HMGB3 Protein.International journal of molecular sciences · 2024
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 5 institutions in 3 countries.

Ari RouhiainenNeuroscience center, University of Helsinki, Finland.
Xiang ZhaoNeuroscience center, University of Helsinki, Finland.
Päivi VanttolaNeuroscience center, University of Helsinki, Finland.
Kui QianInstitute of Biotechnology, University of Helsinki, Finland.
Evgeny KulesskiyNeuroscience center, University of Helsinki, Finland.
Juha Kuja-PanulaNeuroscience center, University of Helsinki, Finland.
Kathleen GransalkeNeuroscience center, University of Helsinki, Finland.
Mikaela GrönholmDepartment of Biosciences, University of Helsinki, Finland.
Emmanual UnniDepartment of Biochemistry, University of Maryland School of Medicine, Baltimore, Maryland, USA.
Marvin MeistrichDepartment of Experimental Radiation Oncology, Division of Radiation Oncology, MD Anderson Cancer Center, Houston, Texas, USA.
Li TianNeuroscience center, University of Helsinki, Finland.
Petri AuvinenInstitute of Biotechnology, University of Helsinki, Finland.
Heikki RauvalaNeuroscience center, University of Helsinki, Finland.
University of Helsinki · FIBeijing HuiLongGuan Hospital · CNInstitute for Molecular Medicine Finland · FIThe University of Texas MD Anderson Cancer Center · USUniversity of Maryland, Baltimore · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

HMGB4 is a new member in the family of HMGB proteins that has been characterized in sperm cells, but little is known about its functions in somatic cells. Here we show that HMGB4 and the highly similar rat Transition Protein 4 (HMGB4L1) are expressed in neuronal cells. Both proteins had slow mobility in nucleus of living NIH-3T3 cells. They interacted with histones and their differential expression in transformed cells of the nervous system altered the post-translational modification statuses of histones in vitro. Overexpression of HMGB4 in HEK 293T cells made cells more susceptible to cell death induced by topoisomerase inhibitors in an oncology drug screening array and altered variant composition of histone H3. HMGB4 regulated over 800 genes in HEK 293T cells with a p-value ≤0.013 (n = 3) in a microarray analysis and displayed strongest association with adhesion and histone H2A -processes. In neuronal and transformed cells HMGB4 regulated the expression of an oligodendrocyte marker gene PPP1R14a and other neuronal differentiation marker genes. In conclusion, our data suggests that HMGB4 is a factor that regulates chromatin and expression of neuronal differentiation markers.

Indexed as

Gene Expression RegulationNeurogenesisAnimalsCell LineChromatinGene Expression ProfilingHigh Mobility Group ProteinsHMGB ProteinsHumansMiceMicroarray AnalysisNeuronsRatsChromatinHigh Mobility Group ProteinsHMGB4 protein, mouseHMGB ProteinsLOC103694517 protein, rat

Identifiers

PMID27608812
PMCPMC5036535
OpenAlexW2509513606

What OpenQuestion holds

Textmetadata
LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.