ArticleScientific reports2016
Probing conformational and functional states of human hepatocyte growth factor by a panel of monoclonal antibodies.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
5 citing papers in PubMed, 10 citations in OpenAlex.
- State of the structure address on MET receptor activation by HGF.Biochemical Society transactions · 2021Review
- Distinct Localization of Mature HGF from its Precursor Form in Developing and Repairing the Stomach.International journal of molecular sciences · 2019Article
- Macrocyclic peptide-based inhibition and imaging of hepatocyte growth factor.Nature chemical biology · 2019Article
- High-throughput retrieval of physical DNA for NGS-identifiable clones in phage display library.mAbs · 2019Article
- Advances in domain and subunit localization technology for electron microscopy.Biophysical reviews · 2019Review
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Authors and funding
9 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
HGF-Met signaling contributes to various biological events by controlling cell migration. Since the abnormal activation of Met receptor causes cancer progression, inhibitors such as neutralizing antibodies are regarded as promising therapeutics. HGF is secreted as a single-chain (sc) precursor and is processed by extracellular proteases to generate disulfide-bonded two-chain (tc) HGF. Although this proteolytic processing of HGF is necessary for its biological activity, exactly how the proteolysis leads to the conversion of HGF to the active form is still unclar due to the lack of structural information. In order to gain insights about this point, we generated 6 antibodies against HGF. All antibodies recognized different epitopes on the native HGF protein and showed distinct effects when tested in a cell-based HGF-Met signaling assay. They included one antibody (t1E4) that strongly blocks Met activation by tcHGF, as well as one antibody (t8E4) exclusively recognizing the active tcHGF but not inactive scHGF. Thus, a panel of anti-HGF antibodies suitable for probing the structural mechanism of HGF activation were obtained.
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Registered trials
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