ArticlePlants (Basel, Switzerland)2016
Knockdown of WHIRLY1 Affects Drought Stress-Induced Leaf Senescence and Histone Modifications of the Senescence-Associated Gene HvS40.
Article in Plants (Basel, Switzerland), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers, 1 of them a synthesis that pooled it.
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Who cites it
28 citing papers in PubMed, 1 synthesis or guideline pooled it, 45 citations in OpenAlex.
- Comparative transcriptome meta-analysis of Arabidopsis thaliana under drought and cold stress.PloS one · 2018Pooled it
- NtWHY1 negatively regulates tobacco cold tolerance via interfering with flavonoid biosynthesis.Plant cell reports · 2026Article
- HOS15 together with PWR-HDA9 positively regulates dark-induced senescence in Arabidopsis.Plant signaling & behavior · 2025Article
- Functional Characterization of thePlants (Basel, Switzerland) · 2025Article
- WHIRLY1 regulates aliphatic glucosinolate biosynthesis in early seedling development of Arabidopsis.The Plant journal : for cell and molecular biology · 2025Article
- WHIRLY proteins, multi-layer regulators linking the nucleus and organelles in developmental and stress-induced senescence of plants.Annals of botany · 2024Review
- Genome-Wide Identification and Molecular Evolutionary History of the Whirly Family Genes inPlants (Basel, Switzerland) · 2024Article
- Genome-Wide Analysis of theInternational journal of molecular sciences · 2024Article
- The ABA-AtNAP-SAG113 PP2C module regulates leaf senescence by dephoshorylating SAG114 SnRK3.25 in Arabidopsis.Molecular horticulture · 2023Article
- Genetic resources and precise gene editing for targeted improvement of barley abiotic stress tolerance.Journal of Zhejiang University. Science. B · 2023Review
- WHIRLY1 Acts Upstream of ABA-Related Reprogramming of Drought-Induced Gene Expression in Barley and Affects Stress-Related Histone Modifications.International journal of molecular sciences · 2023Article
- WHIRLY protein functions in plants.Food and energy security · 2023Review
- Epigenetic control of plant senescence and cell death and its application in crop improvement.Frontiers in plant science · 2023Review
- Identification of Whirly transcription factors in Triticeae species and functional analysis ofFrontiers in plant science · 2023Article
- Genome-wide identification, phylogenetic, and expression analysis under abiotic stress conditions of Whirly (WHY) gene family in Medicago sativa L.Scientific reports · 2022Article
- The Barley Heavy Metal Associated Isoprenylated Plant Protein HvFP1 Is Involved in a Crosstalk between the Leaf Development and Abscisic Acid-Related Drought Stress Responses.Plants (Basel, Switzerland) · 2022Article
- Epigenetic Mechanisms of Senescence in Plants.Cells · 2022Review
- WHIRLIES Are Multifunctional DNA-Binding Proteins With Impact on Plant Development and Stress Resistance.Frontiers in plant science · 2022Review
- Concerto on Chromatin: Interplays of Different Epigenetic Mechanisms in Plant Development and Environmental Adaptation.Plants (Basel, Switzerland) · 2021Review
- Plant-Specific AtS40.4 Acts as a Negative Regulator in Abscisic Acid Signaling During Seed Germination and Seedling Growth inFrontiers in plant science · 2021Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The plastid-nucleus located protein WHIRLY1 has been described as an upstream regulator of leaf senescence, binding to the promoter of senescence-associated genes like HvS40. To investigate the impact of WHIRLY1 on drought stress-induced, premature senescence, transgenic barley plants with an RNAi-mediated knockdown of the HvWHIRLY1 gene were grown under normal and drought stress conditions. The course of leaf senescence in these lines was monitored by physiological parameters and studies on the expression of senescence- and drought stress-related genes. Drought treatment accelerated leaf senescence in WT plants, whereas WHIRLY 1 knockdown lines (RNAi-W1) showed a stay-green phenotype. Expression of both senescence-associated and drought stress-responsive genes, was delayed in the transgenic plants. Notably, expression of transcription factors of the WRKY and NAC families, which are known to function in senescence- and stress-related signaling pathways, was affected in plants with impaired accumulation of WHIRLY1, indicating that WHIRLY1 acts as an upstream regulator of drought stress-induced senescence. To reveal the epigenetic indexing of HvS40 at the onset of drought-induced senescence in WT and RNAi-W1 lines, stress-responsive loading with histone modifications of promoter and coding sequences of HvS40 was analyzed by chromatin immunoprecipitation and quantified by qRT-PCR. In the wildtype, the euchromatic mark H3K9ac of the HvS40 gene was low under control conditions and was established in response to drought treatment, indicating the action of epigenetic mechanisms in response to drought stress. However, drought stress caused no significant increase in H3K9ac in plants impaired in accumulation of WHIRLY1. The results show that WHIRLY1 knockdown sets in motion a delay in senescence that involves all aspects of gene expression, including changes in chromatin structure.
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