ArticleOptics express2016
Light-sheet microscopy with digital Fourier analysis measures transport properties over large field-of-view.
Article in Optics express, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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Who cites it
11 citing papers in PubMed.
- Article
- Millimetric scale two-photon Bessel-Gauss beam light sheet microscopy with three-axis isotropic resolution using an axicon lens.Neurophotonics · 2023Article
- Quantifying Cytoskeleton Dynamics Using Differential Dynamic Microscopy.Journal of visualized experiments : JoVE · 2022Article
- Probing the dynamics of turbid colloidal suspensions using differential dynamic microscopy.Soft matter · 2022Article
- Motor-driven advection competes with crowding to drive spatiotemporally heterogeneous transport in cytoskeleton composites.Frontiers in physics · 2022Article
- Subtle changes in crosslinking drive diverse anomalous transport characteristics in actin-microtubule networks.Soft matter · 2021Article
- Filament Rigidity Vies with Mesh Size in Determining Anomalous Diffusion in Cytoskeleton.Biomacromolecules · 2019Article
- Topology-dependent anomalous dynamics of ring and linear DNA are sensitive to cytoskeleton crosslinking.Science advances · 2019Article
- Bridging the spatiotemporal scales of macromolecular transport in crowded biomimetic systems.Soft matter · 2019Article
- Point-spread function engineering enhances digital Fourier microscopy.Optics letters · 2017Article
- Image windowing mitigates edge effects in Differential Dynamic Microscopy.The European physical journal. E, Soft matter · 2017Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Using light-sheet microscopy combined with digital Fourier methods we probe the dynamics of colloidal samples and DNA molecules. This combination, referred to as selective-plane illumination differential dynamic microscopy (SPIDDM), has the benefit of optical sectioning to study, with minimal photobleaching, thick samples allowing us to measure the diffusivity of colloidal particles at high volume fractions. Further, SPIDDM exploits the inherent spatially-varying thickness of Gaussian light-sheets. Where the excitation sheet is most focused, we capture high spatial frequency dynamics as the signal-to-background is high. In thicker regions, we capture the slower dynamics as diffusion out of the sheet takes longer.
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.