ArticleJournal of parasitic diseases : official organ of the Indian Society for Parasitology2016
Molecular approach for detecting early prepatent Schistosoma mansoni infection in Biomphalaria alexandrina snail host.
Article in Journal of parasitic diseases : official organ of the Indian Society for Parasitology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers, 1 of them a synthesis that pooled it.
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Who cites it
12 citing papers in PubMed, 1 synthesis or guideline pooled it, 16 citations in OpenAlex.
- Prevalence of Schistosoma mansoni and Associated Risk Factors in Human and Biomphalaria Snails in Ethiopia: A Systematic Review and Meta-analysis.Acta parasitologica · 2022Pooled it
- Rapid and Sensitive Detection ofTropical medicine and infectious disease · 2026Article
- Development and field evaluation of a multiplex qPCR assay for environmental DNA detection of Schistosoma mekongi and its intermediate snail host Neotricula aperta in the Mekong River Basin.Infectious diseases of poverty · 2026Article
- Land use drives trematode dynamics in a restored stream system.Current research in parasitology & vector-borne diseases · 2026Article
- Freshwater snail-borne parasitic diseases in Africa.Tropical medicine and health · 2024Review
- Systematic Review and Meta-Analysis on the Infection Rates of Schistosome Transmitting Snails in Southern Africa.Tropical medicine and infectious disease · 2022Review
- A loop-mediated isothermal amplification assay for Schistosoma mansoni detection in Biomphalaria spp. from schistosomiasis-endemic areas in Minas Gerais, Brazil.Parasites & vectors · 2021Article
- Challenges and Opportunities Presented by Current Techniques for Detecting Schistosome Infections in Intermediate Host Snails: A Scoping Review.International journal of environmental research and public health · 2021Article
- Schistosoma species detection by environmental DNA assays in African freshwaters.PLoS neglected tropical diseases · 2020Article
- Environmental DNA-based xenomonitoring for determining Schistosoma presence in tropical freshwaters.Parasites & vectors · 2020Article
- New epidemiological profile of schistosomiasis from an area of low prevalence in Brazil.Revista da Sociedade Brasileira de Medicina Tropical · 2020Article
- Prevalence ofJournal of tropical medicine · 2020Review
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The present study aimed to evaluate a polymerase chain reaction (PCR) assay used for detection of Schistosoma mansoni infection in Biomphalaria alexandrina snails in early prepatent period and to compare between it and the ordinary detection methods (shedding and crushing). Biomphalaria alexandrina snails are best known for their role as intermediate hosts of S. mansoni. DNA was extracted from infected snails in addition to non-infected "negative control" (to optimized the efficiency of PCR reaction) and subjected to PCR using primers specific to a partial sequence of S. mansoni fructose-1,6-bus phosphate aldolase (SMALDO). SMALDO gene was detected in the infected laboratory snails with 70, 85, and 100 % positivity at the 1st, 3rd, and 7th day of infection, respectively. In contrast, the ordinary method was not sensitive enough in detection of early prepatent infection even after 7 days of infection which showed only 25 % positivity. By comparing the sensitivity of the three methods, it was found that the average sensitivity of shedding method compared to PCR was 23.8 % and the average sensitivity of crushing method compared to PCR was 46.4 % while the sensitivity of PCR was 100 %. We conclude that PCR is superior to the conventional methods and can detect positive cases that were negative when examined by shedding or crushing methods. This can help in detection of the areas and times of high transmission which in turn will be very beneficial in planning of the exact timing of the proper control strategy.
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