ArticleMolecular biology of the cell2016
T-lymphocyte passive deformation is controlled by unfolding of membrane surface reservoirs.
Article in Molecular biology of the cell, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 49 citations in OpenAlex.
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- An In Vitro Quantitative Systems Pharmacology Platform for Characterizing CD3-Bispecific Antibody-Mediated T-Cell Activation and Tumor Cell Cytotoxicity.The AAPS journal · 2026Article
- B cell mechanobiology in health and disease: emerging techniques and insights into therapeutic responses.FEBS letters · 2025Review
- Soft matter mechanics of immune cell aggregates.Journal of the Royal Society, Interface · 2025Article
- Microdevice for confinement of T-cells on functionalized bio-interfaces.Lab on a chip · 2025Article
- Cell trajectory modulation: rapid microfluidic biophysical profiling of CAR T cell functional phenotypes.Nature communications · 2025Article
- Plasma membrane folding enables constant surface area-to-volume ratio in growing mammalian cells.Current biology : CB · 2025Article
- Plasma membrane folding enables constant surface area-to-volume ratio in growing mammalian cells.bioRxiv : the preprint server for biology · 2025Article
- Cell-intrinsic mechanical regulation of plasma membrane accumulation at the cytokinetic furrow.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Cell intrinsic mechanical regulation of plasma membrane accumulation at the cytokinetic furrow.bioRxiv : the preprint server for biology · 2024Article
- Quantifying both viscoelasticity and surface tension: Why sharp tips overestimate cell stiffness.Biophysical journal · 2024Article
- Possibilities of using T-cell biophysical biomarkers of ageing.Expert reviews in molecular medicine · 2022Review
- Distinct timing of neutrophil spreading and stiffening during phagocytosis.Biophysical journal · 2022Article
- The spatio-temporal control of effector T cell migration.Nature reviews. Immunology · 2021Review
- A 'dynamic adder model' for cell size homeostasis in Dictyostelium cells.Scientific reports · 2021Article
- Rapid viscoelastic changes are a hallmark of early leukocyte activation.Biophysical journal · 2021Article
- Controlling T cells spreading, mechanics and activation by micropatterning.Scientific reports · 2021Article
- Shigella impairs human T lymphocyte responsiveness by hijacking actin cytoskeleton dynamics and T cell receptor vesicular trafficking.Cellular microbiology · 2020Article
- Regulation of the Total Cell Surface Area in DividingFrontiers in cell and developmental biology · 2020Article
- Hydrodynamics of transient cell-cell contact: The role of membrane permeability and active protrusion length.PLoS computational biology · 2019Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
T-lymphocytes in the human body routinely undergo large deformations, both passively, when going through narrow capillaries, and actively, when transmigrating across endothelial cells or squeezing through tissue. We investigate physical factors that enable and limit such deformations and explore how passive and active deformations may differ. Employing micropipette aspiration to mimic squeezing through narrow capillaries, we find that T-lymphocytes maintain a constant volume while they increase their apparent membrane surface area upon aspiration. Human resting T-lymphocytes, T-lymphoblasts, and the leukemic Jurkat T-cells all exhibit membrane rupture above a critical membrane area expansion that is independent of either micropipette size or aspiration pressure. The unfolded membrane matches the excess membrane contained in microvilli and membrane folds, as determined using scanning electron microscopy. In contrast, during transendothelial migration, a form of active deformation, we find that the membrane surface exceeds by a factor of two the amount of membrane stored in microvilli and folds. These results suggest that internal membrane reservoirs need to be recruited, possibly through exocytosis, for large active deformations to occur.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.