ArticleMolecular biology of the cell2016
Budding yeast chromatin is dispersed in a crowded nucleoplasm in vivo.
Article in Molecular biology of the cell, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed.
- The shifting paradigm of chromatin structure: from the 30-nm chromatin fiber to liquid-like organization.Proceedings of the Japan Academy. Series B, Physical and biological sciences · 2025Review
- Nanoscale analysis of human G1 and metaphase chromatin in situ.The EMBO journal · 2025Article
- ChromEMT: visualizing and reconstructing chromatin ultrastructure and 3D organization in situ.Nature protocols · 2025Review
- nucMACC: An MNase-seq pipeline to identify structurally altered nucleosomes in the genome.Science advances · 2024Article
- Structure of native chromatin fibres revealed by Cryo-ET in situ.Nature communications · 2023Article
- Single-molecule study reveals Hmo1, not Hho1, promotes chromatin assembly in budding yeast.mBio · 2023Article
- Article
- Condensed but liquid-like domain organization of active chromatin regions in living human cells.Science advances · 2023Article
- Single-nucleosome imaging reveals steady-state motion of interphase chromatin in living human cells.Science advances · 2022Article
- Article
- Phospho-Tau and Chromatin Landscapes in Early and Late Alzheimer's Disease.International journal of molecular sciences · 2021Review
- Tetranucleosome Interactions Drive Chromatin Folding.ACS central science · 2021Article
- Nucleosome plasticity is a critical element of chromatin liquid-liquid phase separation and multivalent nucleosome interactions.Nature communications · 2021Article
- Physical Nature of Chromatin in the Nucleus.Cold Spring Harbor perspectives in biology · 2021Review
- Visualization of Chromatin in the Yeast Nucleus and Nucleolus Using Hyperosmotic Shock.International journal of molecular sciences · 2021Article
- Global histone protein surface accessibility in yeast indicates a uniformly loosely packed genome with canonical nucleosomes.Epigenetics & chromatin · 2021Article
- A method for assessing histone surface accessibility genome-wide.Methods (San Diego, Calif.) · 2020Article
- Cryo-nanoscale chromosome imaging-future prospects.Biophysical reviews · 2020Review
- Biology and Physics of Heterochromatin-Cells · 2020Review
- Liquid-like interactions in heterochromatin: Implications for mechanism and regulation.Current opinion in cell biology · 2020Review
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7 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Chromatin organization has an important role in the regulation of eukaryotic systems. Although recent studies have refined the three-dimensional models of chromatin organization with high resolution at the genome sequence level, little is known about how the most fundamental units of chromatin-nucleosomes-are positioned in three dimensions in vivo. Here we use electron cryotomography to study chromatin organization in the budding yeast Saccharomyces cerevisiae Direct visualization of yeast nuclear densities shows no evidence of 30-nm fibers. Aside from preribosomes and spindle microtubules, few nuclear structures are larger than a tetranucleosome. Yeast chromatin does not form compact structures in interphase or mitosis and is consistent with being in an "open" configuration that is conducive to high levels of transcription. From our study and those of others, we propose that yeast can regulate its transcription using local nucleosome-nucleosome associations.
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