ArticleScientific reports2016
Site-specific fluorescent labeling to visualize membrane translocation of a myristoyl switch protein.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
10 citing papers in PubMed, 13 citations in OpenAlex.
- Enhanced Site-Specific Fluorescent Labeling of Membrane Proteins Using Native Nanodiscs.Biomolecules · 2025Article
- Genetically Engineered Liposwitch-Based Nanomaterials.Biomacromolecules · 2024Article
- Site-Specific Labeling of Endogenous Proteins Using CoLDR Chemistry.Journal of the American Chemical Society · 2021Article
- Regulation of retinal membrane guanylyl cyclase (RetGC) by negative calcium feedback and RD3 protein.Pflugers Archiv : European journal of physiology · 2021Review
- Transport Properties of Gramicidin A Ion Channel in a Free-Standing Lipid Bilayer Filled With Oil Inclusions.Frontiers in cell and developmental biology · 2020Article
- Conductance Changes in Bovine Serum Albumin Caused by Drug-Binding Triggered Structural Transitions.Materials (Basel, Switzerland) · 2019Article
- Novel approaches to probe the binding of recoverin to membranes.European biophysics journal : EBJ · 2018Article
- The Binding Properties and Physiological Functions of Recoverin.Frontiers in molecular neuroscience · 2018Review
- Zebrafish Recoverin Isoforms Display Differences in Calcium Switch Mechanisms.Frontiers in molecular neuroscience · 2018Article
- Highly chromophoric Cy5-methionine for N-terminal fluorescent tagging of proteins in eukaryotic translation systems.Scientific reports · 2017Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Fluorescence approaches have been widely used for elucidating the dynamics of protein-membrane interactions in cells and model systems. However, non-specific multi-site fluorescent labeling often results in a loss of native structure and function, and single cysteine labeling is not feasible when native cysteines are required to support a protein's folding or catalytic activity. Here, we develop a method using genetic incorporation of non-natural amino acids and bio-orthogonal chemistry to site-specifically label with a single fluorescent small molecule or protein the myristoyl-switch protein recoverin, which is involved in rhodopsin-mediated signaling in mammalian visual sensory neurons. We demonstrate reversible Ca(2+)-responsive translocation of labeled recoverin to membranes and show that recoverin favors membranes with negative curvature and high lipid fluidity in complex heterogeneous membranes, which confers spatio-temporal control over down-stream signaling events. The site-specific orthogonal labeling technique is promising for structural, dynamical, and functional studies of many lipid-anchored membrane protein switches.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.