Evidence map›Paper›PMID 27604701›Full record

ArticleClinical rheumatology2017

Absence of mycobacterial DNA in peripheral blood and artery specimens in patients with Takayasu arteritis.

Evanir S Carvalho, Alexandre W S de Souza, Sylvia Cardoso Leão, Maurício Levy-Neto, Rosangela Siqueira de Oliveira, Wonder Drake, Marcello Fabiano de Franco, Paulo H N Saldiva, Paulo Sampaio Gutierrez, Luís Eduardo C Andrade

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Clinical rheumatology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed, 2 pooled it
2.8field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 2 syntheses or guidelines pooled it, 21 citations in OpenAlex.

  1. Pooled it
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  7. Review
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  12. Cutaneous Manifestations of Medium- and Large-Vessel Vasculitis.Clinical reviews in allergy & immunology · 2017
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 5 institutions in 2 countries.

Evanir S CarvalhoUniversidade Federal de São Paulo, São Paulo, SP, Brazil.
Alexandre W S de SouzaUniversidade Federal de São Paulo, São Paulo, SP, Brazil.
Sylvia Cardoso LeãoUniversidade Federal de São Paulo, São Paulo, SP, Brazil.
Maurício Levy-NetoUniversidade de São Paulo, São Paulo, SP, Brazil.
Rosangela Siqueira de OliveiraInstituto Adolfo Lutz, São Paulo, SP, Brazil.
Wonder DrakeVanderbilt University, Nashville, TN, USA.
Marcello Fabiano de FrancoUniversidade Federal de São Paulo, São Paulo, SP, Brazil.
Paulo H N SaldivaUniversidade de São Paulo, São Paulo, SP, Brazil.
Paulo Sampaio GutierrezInstituto do Coração (INCOR), São Paulo, SP, Brazil.
Luís Eduardo C AndradeUniversidade Federal de São Paulo, São Paulo, SP, Brazil. luis.andrade@unifesp.br.
Universidade Federal de São Paulo · BRUniversidade de São Paulo · BRHospital do Coração · BRInstituto Adolfo Lutz · BRVanderbilt University · US

Funding

Microbial induction of sarcoidosis CD4+ T cell dysfunctionR01HL117074 · NHLBI · VANDERBILT UNIVERSITY MEDICAL CENTER · PI BERNARD, GORDON RAPHAEL, DRAKE, WONDER P. · 2013 to 2017
$3.8M
Mentoring in Translational Research in Interstitial Lung DiseasesK24HL127301 · NHLBI · VANDERBILT UNIVERSITY MEDICAL CENTER · PI Wonder P. Drake · 2016 to 2026
$1.2M
NHLBI NIH HHS K24 HL127301NHLBI NIH HHS R01 HL117074
6 · The paper itself

Abstract

The objective of this study was to demonstrate the presence of mycobacterial nucleic acid sequences in peripheral blood and arteries from patients with Takayasu arteritis (TA). Polymerase chain reaction was performed to detect mycobacterial DNA from three different nucleic acid sequences including the insertion sequence (IS) 6110, the 65-kDa heat shock protein gene (HSP65), and the 16S ribosomal RNA (rRNA) gene in peripheral blood from 32 TA patients and in arterial specimens from 10 TA patients. Twenty-eight HIV-negative patients with pulmonary tuberculosis prior to therapy were tested for IS6110 in peripheral blood as positive controls, and 24 blood donors were evaluated as healthy controls (HC). All TA patients were negative for the insertion sequence IS6110 and for HSP65 and 16S rRNA genes in blood samples and in arterial specimens. IS6110 sequence was found in peripheral blood from 22 (78.5 %) patients with pulmonary tuberculosis but not in HC. In conclusion, the strategy of mycobacterial-specific nucleic acid amplification in the peripheral blood and arterial specimens of TA patients was unable to lend support to the association between TA and tuberculosis long suggested in the literature.

Indexed as

AdolescentAdultArteriesBacterial ProteinsCase-Control StudiesChaperonin 60DNA, BacterialFemaleHumansMaleMiddle AgedMycobacterium tuberculosisPolymerase Chain ReactionRNA, Ribosomal, 16STakayasu ArteritisTuberculosis, PulmonaryBacterial ProteinsChaperonin 60DNA, Bacterialheat-shock protein 65, MycobacteriumRNA, Ribosomal, 16SDNAMycobacterium tuberculosisPolymerase chain reactionTakayasu arteritis

Identifiers

PMID27604701
PMCPMC5742422
OpenAlexW2517626374

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.