ArticleScientific reports2016
Biosynthesis and Regulation of Wheat Amylose and Amylopectin from Proteomic and Phosphoproteomic Characterization of Granule-binding Proteins.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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26 citing papers in PubMed, 66 citations in OpenAlex.
- MGIDI-based ideotype selection and genetic variation for wheat agronomic traits and bread-making quality (BMQ) under drought stress condition.BMC plant biology · 2026Article
- Subclass III SnRK2 Kinases Coordinate Starch and Storage Protein Synthesis During Maize Kernel Development.Plant biotechnology journal · 2026Article
- Australian Hard 2 (AH2) wheat class with null4A genetic basis to produce high-quality Japanese ramen noodle.Journal of the science of food and agriculture · 2026Article
- Effects of a novel loss-of-function waxy endosperm allele on sorghum seed development and grain quality.G3 (Bethesda, Md.) · 2025Article
- Impact of Temperature Stresses on Wheat Quality: A Focus on Starch and Protein Composition.Foods (Basel, Switzerland) · 2025Review
- Genome-wide association study and KASP marker development for starch quality traits in wheat.The plant genome · 2024Article
- Comparative Proteomic Analysis of Wild and Cultivated Amaranth Species Seeds by 2-DE and ESI-MS/MS.Plants (Basel, Switzerland) · 2024Article
- Exploring a novel GH13_5 α-amylase from Jeotgalibacillus malaysiensis D5AMB Express · 2024Article
- Wheat-Based Glues in Conservation and Cultural Heritage: (Dis)solving the Proteome of Flour and Starch Pastes and Their Adhering Properties.Journal of proteome research · 2024Article
- Bioactive Edible Gel Films Based on Wheat Flour and Glucose for Food Packaging Applications.Gels (Basel, Switzerland) · 2024Article
- Resistant starch formation in rice: Genetic regulation and beyond.Plant communications · 2022Review
- Starch synthases SSIIa and GBSSI control starch structure but do not determine starch granule morphology in the absence of SSIIIa and SSIVb.Plant molecular biology · 2022Article
- Characterization of starch structures isolated from the grains of waxy, sweet, and hybrid sorghum (Frontiers in nutrition · 2022Article
- Review
- Comparative Phosphoproteomic Analysis Reveals the Response of Starch Metabolism to High-Temperature Stress in Rice Endosperm.International journal of molecular sciences · 2021Article
- Proteomics and Post-Translational Modifications of Starch Biosynthesis-Related Proteins in Developing Seeds of Rice.International journal of molecular sciences · 2021Review
- Posttranslational Modification of Waxy to Genetically Improve Starch Quality in Rice Grain.International journal of molecular sciences · 2021Review
- Review
- Identification and molecular characterization of mutant line deficiency in three waxy proteins of common wheat (Triticum aestivum L.).Scientific reports · 2021Article
- Article
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Waxy starch has an important influence on the qualities of breads. Generally, grain weight and yield in waxy wheat (Triticum aestivum L.) are significantly lower than in bread wheat. In this study, we performed the first proteomic and phosphoproteomic analyses of starch granule-binding proteins by comparing the waxy wheat cultivar Shannong 119 and the bread wheat cultivar Nongda 5181. These results indicate that reduced amylose content does not affect amylopectin synthesis, but it causes significant reduction of total starch biosynthesis, grain size, weight and grain yield. Two-dimensional differential in-gel electrophoresis identified 40 differentially expressed protein (DEP) spots in waxy and non-waxy wheats, which belonged mainly to starch synthase (SS) I, SS IIa and granule-bound SS I. Most DEPs involved in amylopectin synthesis showed a similar expression pattern during grain development, suggesting relatively independent amylose and amylopectin synthesis pathways. Phosphoproteome analysis of starch granule-binding proteins, using TiO2 microcolumns and LC-MS/MS, showed that the total number of phosphoproteins and their phosphorylation levels in ND5181 were significantly higher than in SN119, but proteins controlling amylopectin synthesis had similar phosphorylation levels. Our results revealed the lack of amylose did not affect the expression and phosphorylation of the starch granule-binding proteins involved in amylopectin biosynthesis.
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