ArticleBMC genomics2016
High throughput techniques to reveal the molecular physiology and evolution of digestion in spiders.
Article in BMC genomics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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21 citing papers in PubMed, 38 citations in OpenAlex.
- Comparative analysis of venom genes in the chromosome-level genomes of two closely related cone snails.BMC genomics · 2026Article
- Exploring Spiders Without Venom as New Sources of Peptidase Inhibitors.International journal of molecular sciences · 2025Article
- Digestion in the arachnid Mischonyx squalidus as a probable source of lipids to synthesize opiliones defense and communication molecules.Scientific reports · 2025Article
- Beyond venomous fangs: Uloboridae spiders have lost their venom but not their toxicity.BMC biology · 2025Article
- The proteomic content of Varroa destructor gut varies according to the developmental stage of its host.PLoS pathogens · 2024Article
- Enlightening the toxinological dark matter of spider venom enzymes.npj biodiversity · 2024Article
- Article
- Spider-Venom Peptides: Structure, Bioactivity, Strategy, and Research Applications.Molecules (Basel, Switzerland) · 2023Review
- Digestive enzymes and sphingomyelinase D in spiders without venom (Uloboridae).Scientific reports · 2023Article
- Spiders' digestive system as a source of trypsin inhibitors: functional activity of a member of atracotoxin structural family.Scientific reports · 2023Article
- Review
- Toxinology in the proteomics era: a review on arachnid venom proteomics.The journal of venomous animals and toxins including tropical diseases · 2022Article
- Linking toxicity and predation in a venomous arthropod: the case ofThe journal of venomous animals and toxins including tropical diseases · 2022Article
- Venomics of the ectoparasitoid wasp Bracon nigricans.BMC genomics · 2020Article
- Not so Dangerous After All? Venom Composition and Potency of the Pholcid (Daddy Long-Leg) SpiderFrontiers in ecology and evolution · 2019Article
- Article
- The gut epithelium from feeding to fasting in the predatory soil mite Pergamasus longicornis (Mesostigmata: Parasitidae): one tissue, two roles.Experimental & applied acarology · 2019Review
- Venomics: A Mini-Review.High-throughput · 2018Review
- A Multifaceted Mass Spectrometric Method to Probe Feeding Related Neuropeptide Changes in Callinectes sapidus and Carcinus maenas.Journal of the American Society for Mass Spectrometry · 2018Article
- Characterisation of protein families in spider digestive fluids and their role in extra-oral digestion.BMC genomics · 2017Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundSpiders are known for their predatory efficiency and for their high capacity of digesting relatively large prey. They do this by combining both extracorporeal and intracellular digestion. Whereas many high throughput ("-omics") techniques focus on biomolecules in spider venom, so far this approach has not yet been applied to investigate the protein composition of spider midgut diverticula (MD) and digestive fluid (DF).
resultsWe here report on our investigations of both MD and DF of the spider Nephilingis (Nephilengys) cruentata through the use of next generation sequencing and shotgun proteomics. This shows that the DF is composed of a variety of hydrolases including peptidases, carbohydrases, lipases and nuclease, as well as of toxins and regulatory proteins. We detect 25 astacins in the DF. Phylogenetic analysis of the corresponding transcript(s) in Arachnida suggests that astacins have acquired an unprecedented role for extracorporeal digestion in Araneae, with different orthologs used by each family. The results of a comparative study of spiders in distinct physiological conditions allow us to propose some digestion mechanisms in this interesting animal taxon.
conclusionAll the high throughput data allowed the demonstration that DF is a secretion originating from the MD. We identified enzymes involved in the extracellular and intracellular phases of digestion. Besides that, data analyses show a large gene duplication event in Araneae digestive process evolution, mainly of astacin genes. We were also able to identify proteins expressed and translated in the digestive system, which until now had been exclusively associated to venom glands.
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