ArticlePloS one2016
Demonstration of Protein-Based Human Identification Using the Hair Shaft Proteome.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
31 citing papers in PubMed, 1 synthesis or guideline pooled it, 147 citations in OpenAlex.
- Ethical Principles, Constraints and Opportunities in Clinical Proteomics.Molecular & cellular proteomics : MCP · 2021Pooled it
- Enhanced Genetically Variant Peptide Profiling from Human Hair Using Multiple Enzymes.ACS omega · 2026Article
- The multifaceted role of hair as a biospecimen: recent advances in precision medicine and forensic science.Experimental & molecular medicine · 2025Review
- Advantages of Incomplete Digestion in Human Hair Shaft Proteomics, a Focus on Cuticular Keratins.Rapid communications in mass spectrometry : RCM · 2025Article
- DNA forensics at forty: the way forward.International journal of legal medicine · 2025Review
- Hair Metabolomic Profiling of Diseased Forest Musk Deer (Animals : an open access journal from MDPI · 2025Article
- A multi-level overview of the hair decomposition process.International journal of legal medicine · 2025Review
- Advances in the Study of Protein Deamidation: Unveiling Its Influence on Aging, Disease Progression, Forensics and Therapeutic Efficacy.Proteomes · 2025Review
- Proteomics of the Dark-Ventral-Patch Sexual Signal in Male Red Deer.Animals : an open access journal from MDPI · 2025Article
- RedefiningFrontiers in microbiology · 2025Review
- Proteomic Analysis of Single Hairs.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Starch treatment improves the salivary proteome for subject identification purposes.Forensic science, medicine, and pathology · 2024Article
- Proteins in scalp hair of preschool children.Psych · 2024Article
- Improved Sample Preparation Method for Protein and Peptide Identification from Human Hair.Journal of proteome research · 2024Article
- Age-Related Changes in Post-Translational Modifications of Proteins from Whole Male and Female Skeletal Elements.Molecules (Basel, Switzerland) · 2023Article
- DNA and protein analyses of hair in forensic genetics.International journal of legal medicine · 2023Review
- Peptide ancestry informative markers in uterine neoplasms from women of European, African, and Asian ancestry.iScience · 2022Article
- Identifying individuals using proteomics: are we there yet?Frontiers in molecular biosciences · 2022Review
- On the Identification of Body Fluids and Tissues: A Crucial Link in the Investigation and Solution of Crime.Genes · 2021Review
- Creation of a peptide database of corneous beta-proteins of marine turtles for the identification of tortoiseshell: archaeological combs as case study.Royal Society open science · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
18 authors at 7 institutions in 2 countries.
Funding
Abstract
Human identification from biological material is largely dependent on the ability to characterize genetic polymorphisms in DNA. Unfortunately, DNA can degrade in the environment, sometimes below the level at which it can be amplified by PCR. Protein however is chemically more robust than DNA and can persist for longer periods. Protein also contains genetic variation in the form of single amino acid polymorphisms. These can be used to infer the status of non-synonymous single nucleotide polymorphism alleles. To demonstrate this, we used mass spectrometry-based shotgun proteomics to characterize hair shaft proteins in 66 European-American subjects. A total of 596 single nucleotide polymorphism alleles were correctly imputed in 32 loci from 22 genes of subjects' DNA and directly validated using Sanger sequencing. Estimates of the probability of resulting individual non-synonymous single nucleotide polymorphism allelic profiles in the European population, using the product rule, resulted in a maximum power of discrimination of 1 in 12,500. Imputed non-synonymous single nucleotide polymorphism profiles from European-American subjects were considerably less frequent in the African population (maximum likelihood ratio = 11,000). The converse was true for hair shafts collected from an additional 10 subjects with African ancestry, where some profiles were more frequent in the African population. Genetically variant peptides were also identified in hair shaft datasets from six archaeological skeletal remains (up to 260 years old). This study demonstrates that quantifiable measures of identity discrimination and biogeographic background can be obtained from detecting genetically variant peptides in hair shaft protein, including hair from bioarchaeological contexts.
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Registered trials
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