ArticleOncotarget2016
Epigenome-wide association study of smoking and DNA methylation in non-small cell lung neoplasms.
Article in Oncotarget, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 2 of them syntheses that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 2 syntheses or guidelines pooled it, 31 citations in OpenAlex.
- Appraising the causal relevance of DNA methylation for risk of lung cancer.International journal of epidemiology · 2019Pooled it
- Clinical significance of miRNA‑1 and its potential target gene network in lung squamous cell carcinoma.Molecular medicine reports · 2019Pooled it
- Epigenetic signatures of starting and stopping smoking.EBioMedicine · 2018Trial
- Article
- Identification of ALDH7A1 as a DNA-methylation-driven gene in lung squamous cell carcinoma.Annals of medicine · 2025Article
- Genomic imprinting biomarkers for cervical cancer risk stratification.Cancer communications (London, England) · 2024Article
- Comprehensive evaluation of smoking exposures and their interactions on DNA methylation.EBioMedicine · 2024Article
- Non-small Cell Lung Cancer Epigenomes Exhibit Altered DNA Methylation in Smokers and Never-smokers.Genomics, proteomics & bioinformatics · 2023Article
- DNA Methylation Abnormalities and Altered Whole Transcriptome Profiles after Switching from Combustible Tobacco Smoking to Heated Tobacco Products.Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology · 2022Article
- A Final Frontier in Environment-Genome Interactions? Integrated, Multi-Omic Approaches to Predictions of Non-Communicable Disease Risk.Frontiers in genetics · 2022Review
- Reduced ribosomal RNA expression and unchanged ribosomal DNA promoter methylation in oral squamous cell carcinoma.Molecular genetics & genomic medicine · 2019Article
- Active and secondhand smoke exposure throughout life and DNA methylation in breast tumors.Cancer causes & control : CCC · 2019Article
- Smoking induces coordinated DNA methylation and gene expression changes in adipose tissue with consequences for metabolic health.Clinical epigenetics · 2018Article
- An integrated -omics analysis of the epigenetic landscape of gene expression in human blood cells.BMC genomics · 2018Article
- Genomic and Bioinformatics Approaches for Analysis of Genes Associated With Cancer Risks Following Exposure to Tobacco Smoking.Frontiers in public health · 2018Review
- Tobacco biomarkers and genetic/epigenetic analysis to investigate ethnic/racial differences in lung cancer risk among smokers.NPJ precision oncology · 2018Review
- DNA methylation in lung tissues of mouse offspring exposed in utero to polycyclic aromatic hydrocarbons.Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association · 2017Article
- Promoter DNA methylation analysis reveals a combined diagnosis of CpG-based biomarker for prostate cancer.Oncotarget · 2017Article
- Association of Smoking, Alcohol Use, and Betel Quid Chewing with Epigenetic Aberrations in Cancers.International journal of molecular sciences · 2017Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Tobacco smoke is a well-established lung cancer carcinogen. We hypothesize that epigenetic processes underlie carcinogenesis. The objective of this study is to examine the effects of smoke exposure on DNA methylation to search for novel susceptibility loci. We obtained epigenome-wide DNA methylation data from lung adenocarcinoma (LUAD) and lung squamous cell (LUSC) tissues in The Cancer Genome Atlas (TCGA). We performed a two-stage discovery (n = 326) and validation (n = 185) analysis to investigate the association of epigenetic DNA methylation level with cigarette smoking pack-years. We also externally validated our findings in an independent dataset. Linear model with least square estimator and spline regression were performed to examine the association between DNA methylation and smoking. We identified five CpG sites highly associated with pack-years of cigarette smoking. Smoking was negatively associated with methylation levels in cg25771041 (WWTR1, p = 3.6 × 10-9), cg16200496 (NFIX, p = 3.4 × 10-12), cg22515201 (PLA2G6, p = 1.0 × 10-9) and cg24823993 (NHP2L1, p = 5.1 × 10-8) and positively associated with the methylation level in cg11875268 (SMUG1, p = 4.3 × 10-8). The CpG-smoking association was stronger in LUSC than LUAD. Of the five loci, smoking explained the most variation in cg16200496 (R2 = 0.098 [both types] and 0.144 [LUSC]). We identified 5 novel CpG candidates that demonstrate differential methylation patterns associated with smoke exposure in lung neoplasms.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.