ArticleOncotarget2016
Quantification of nucleic acid quality in postmortem tissues from a cancer research autopsy program.
Article in Oncotarget, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Intrapatient tumour heterogeneity and clonal evolution in an autopsy study of metastatic salivary gland cancer.Molecular oncology · 2026Article
- DNA-Based Boolean Logic Gates for Molecular Computation and Biosensing: A Critical Review.Molecular biotechnology · 2026Review
- [Half a century of gene expression analysis : The path to modern spatial transcriptomics in autopsy tissue].Pathologie (Heidelberg, Germany) · 2026Review
- Dynamics of Postmortem Gene Expression in Normal and Neoplastic Murine Liver.Life (Basel, Switzerland) · 2026Article
- Optimization and comparison of genomic DNA extraction from whole blood collected in PAXgene blood RNA tube using automated platforms.Clinical chemistry and laboratory medicine · 2026Article
- An analysis of RNA quality metrics in human brain tissue.Journal of neuropathology and experimental neurology · 2025Article
- Rapid autopsies to enhance metastatic research: the UPTIDER post-mortem tissue donation program.NPJ breast cancer · 2024Article
- Blood taken immediately after fatal resuscitation attempts yields higher quality DNA for genetic studies as compared to autopsy samples.International journal of legal medicine · 2023Article
- Proteome alterations in human autopsy tissues in relation to time after death.Cellular and molecular life sciences : CMLS · 2023Article
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- Post-mortem tissue donation programs as platforms to accelerate cancer research.The journal of pathology. Clinical research · 2020Review
- Hepatic gene expression explains primary drug toxicity in bipolar disorder.Translational psychiatry · 2019Article
- Cancer biology as revealed by the research autopsy.Nature reviews. Cancer · 2019Review
- A survey of health care professionals and oncology patients at the McGill University Health Centre reveals enthusiasm for establishing a postmortem rapid tissue donation program.Current oncology (Toronto, Ont.) · 2019Article
- Establishing and Maintaining an Extensive Library of Patient-Derived Xenograft Models.Frontiers in oncology · 2018Article
- Systematic analysis of gene expression patterns associated with postmortem interval in human tissues.Scientific reports · 2017Article
Corrections and comments
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Authors and funding
9 authors.
Funding
Abstract
The last decade has seen a marked rise in the use of cancer tissues obtained from research autopsies. Such resources have been invaluable for studying cancer evolution or the mechanisms of therapeutic resistance to targeted therapies. Degradation of biomolecules is a potential challenge to usage of cancer tissues obtained in the post-mortem setting and remains incompletely studied. We analysed the nucleic acid quality in 371 different frozen tissue samples collected from 80 patients who underwent a research autopsy, including eight normal tissue types, primary and metastatic tumors. Our results indicate that RNA integrity number (RIN) of normal tissues decline with the elongation of post-mortem interval (PMI) in a tissue-type specific manner. Unlike normal tissues, the RNA quality of cancer tissues is highly variable with respect to post-mortem interval. The kinetics of DNA damage also has tissue type-specific features. Moreover, while DNA degradation is an indicator of low RNA quality, the converse is not true. Finally, we show that despite RIN values as low as 5.0, robust data can be obtained by RNA sequencing that reliably discriminates expression signatures.
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Registered trials
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