ArticlePeerJ2016
An optimized approach to germ-free rearing in the jewel wasp Nasonia.
Article in PeerJ, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
12 citing papers in PubMed, 24 citations in OpenAlex.
- Food provisioning to Pardosa spiders decreases the levels of tissue-resident endosymbiotic bacteria.Scientific reports · 2023Article
- Genome of the parasitoid wasp Cotesia chilonis sheds light on amino acid resource exploitation.BMC biology · 2022Article
- An optimized method for Nasonia germ-free rearing.Scientific reports · 2022Article
- Article
- When your host shuts down: larval diapause impacts host-microbiome interactions in Nasonia vitripennis.Microbiome · 2021Article
- Genomes of Gut Bacteria frommSystems · 2021Article
- Article
- Ecological and evolutionary mechanisms underlying patterns of phylosymbiosis in host-associated microbial communities.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2020Review
- Genetic manipulation allows in vivo tracking of the life cycle of the son-killer symbiont, Arsenophonus nasoniae, and reveals patterns of host invasion, tropism and pathology.Environmental microbiology · 2019Article
- Phylosymbiosis Impacts Adaptive Traits inmBio · 2019Article
- Phylosymbiosis: Relationships and Functional Effects of Microbial Communities across Host Evolutionary History.PLoS biology · 2016Article
- Disentangling a Holobiont - Recent Advances and Perspectives inFrontiers in microbiology · 2016Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Development of a Nasonia in vitrogerm-free rearing system in 2012 enabled investigation of Nasonia-microbiota interactions and real-time visualization of parasitoid metamorphosis. However, the use of antibiotics, bleach, and fetal bovine serum introduced artifacts relative to conventional rearing of Nasonia. Here, we optimize the germ-free rearing procedure by using filter sterilization in lieu of antibiotics and by removing residual bleach and fetal bovine serum. Comparison of these methods reveals no influence on larval survival or growth, and a 52% improvement in adult production. Additionally, adult males produced in the new germ-free system are similar in size to conventionally reared males. Experimental implications of these changes are discussed.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.