ArticleStandards in genomic sciences2016
The complete genome sequence of the methanogenic archaeon ISO4-H5 provides insights into the methylotrophic lifestyle of a ruminal representative of the Methanomassiliicoccales.
Article in Standards in genomic sciences, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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23 citing papers in PubMed, 50 citations in OpenAlex.
- Nitro- and nitrooxy-organic inhibitors of methanogenesis: revealing knowledge gaps and alternate ways in bovine rumen microbiome metabolism.Applied and environmental microbiology · 2026Article
- Effects of coenzyme M and methyl-coenzyme M on the efficacy of inhibitors of methanogenesis in rumen cultures.Scientific reports · 2026Article
- Cultivation and sequencing of microbiota members unveil the functional potential of yak gut microbiota.mSystems · 2025Article
- Metataxonomic and metabolomic profiling revealed Pinus koraiensis cone essential oil reduced methane emission through affecting ruminal microbial interactions and host-microbial metabolism.Animal microbiome · 2024Article
- Unraveling the phylogenomic diversity of Methanomassiliicoccales and implications for mitigating ruminant methane emissions.Genome biology · 2024Article
- Structural and functional insights from the sequences and complex domain architecture of adhesin-like proteins fromFrontiers in microbiology · 2024Article
- Article
- Evolving understanding of rumen methanogen ecophysiology.Frontiers in microbiology · 2023Review
- Full Genome Sequence of aMicrobiology resource announcements · 2021Article
- The rumen microbiome inhibits methane formation through dietary choline supplementation.Scientific reports · 2021Article
- Comparative genomic analysis of Methanimicrococcus blatticola provides insights into host adaptation in archaea and the evolution of methanogenesis.ISME communications · 2021Article
- Effect of Methane Inhibitors on Ruminal Microbiota During Early Life and Its Relationship With Ruminal Metabolism and Growth in Calves.Frontiers in microbiology · 2021Article
- New Insights into the Ecology and Physiology ofMicroorganisms · 2020Article
- Synthetic Sequencing Standards: A Guide to Database Choice for Rumen Microbiota Amplicon Sequencing Analysis.Frontiers in microbiology · 2020Article
- A Comparison of Two DNA Metagenomic Bioinformatic Pipelines While Evaluating the Microbial Diversity in Feces of Tanzanian Small Holder Dairy Cattle.BioMed research international · 2020Article
- Uncovering the Diversity and Activity of Methylotrophic Methanogens in Freshwater Wetland Soils.mSystems · 2019Article
- Different response of bacteria, archaea and fungi to process parameters in nine full-scale anaerobic digesters.Microbial biotechnology · 2019Article
- Diverse hydrogen production and consumption pathways influence methane production in ruminants.The ISME journal · 2019Article
- Methane Emission, Rumen Fermentation, and Microbial Community Response to a Nitrooxy Compound in Low-Quality Forage Fed Hu Sheep.Current microbiology · 2019Article
- tRNARNA biology · 2018Review
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11 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Methane emissions from agriculture represent around 9 % of global anthropogenic greenhouse emissions. The single largest source of this methane is animal enteric fermentation, predominantly from ruminant livestock where it is produced mainly in their fermentative forestomach (or reticulo-rumen) by a group of archaea known as methanogens. In order to reduce methane emissions from ruminants, it is necessary to understand the role of methanogenic archaea in the rumen, and to identify their distinguishing characteristics that can be used to develop methane mitigation technologies. To gain insights into the role of methylotrophic methanogens in the rumen environment, the genome of a methanogenic archaeon has been sequenced. This isolate, strain ISO4-H5, was isolated from the ovine rumen and belongs to the order Methanomassiliicoccales. Genomic analysis suggests ISO4-H5 is an obligate hydrogen-dependent methylotrophic methanogen, able to use methanol and methylamines as substrates for methanogenesis. Like other organisms within this order, ISO4-H5 does not possess genes required for the first six steps of hydrogenotrophic methanogenesis. Comparison between the genomes of different members of the order Methanomassiliicoccales revealed strong conservation in energy metabolism, particularly in genes of the methylotrophic methanogenesis pathway, as well as in the biosynthesis and use of pyrrolysine. Unlike members of Methanomassiliicoccales from human sources, ISO4-H5 does not contain the genes required for production of coenzyme M, and so likely requires external coenzyme M to survive.
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