ArticleReproduction (Cambridge, England)2016
Sex differences in response of the bovine embryo to colony-stimulating factor 2.
Article in Reproduction (Cambridge, England), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
22 citing papers in PubMed, 37 citations in OpenAlex.
- Bovine embryo productionAnimal reproduction · 2024Review
- Disruption of CSF2RA in the bovine preimplantation embryo reduces development and affects embryonic gene expression in utero.Reproduction & fertility · 2023Article
- Heparin and Progesterone Exert Synergistic Effects to Improve the In-Vitro Fertilization Rate of Bovine Sperm Bound to Oviduct Cell Aggregates from the Isthmus.Veterinary sciences · 2022Article
- Bioactive supplements influencing bovine in vitro embryo development.Journal of animal science · 2022Review
- Actions of CSF2 and DKK1 on bovine embryo development and pregnancy outcomes are affected by composition of embryo culture medium.Scientific reports · 2022Article
- Cytokines That Serve as Embryokines in Cattle.Animals : an open access journal from MDPI · 2021Review
- Culture Medium and Sex Drive Epigenetic Reprogramming in Preimplantation Bovine Embryos.International journal of molecular sciences · 2021Article
- Regulation of gene expression in the bovine blastocyst by colony-stimulating factor 2 is disrupted by CRISPR/Cas9-mediated deletion of CSF2RA.Biology of reproduction · 2021Article
- An improved method for specific-target preamplification PCR analysis of single blastocysts useful for embryo sexing and high-throughput gene expression analysis.Journal of dairy science · 2021Article
- Actions of putative embryokines on development of the preimplantation bovine embryo to the blastocyst stage.Journal of dairy science · 2020Article
- Actions of colony-stimulating factor 3 on the maturing oocyte and developing embryo in cattle.Journal of animal science · 2020Article
- Molecular fingerprint of female bovine embryos produced in vitro with high competence to establish and maintain pregnancy†.Biology of reproduction · 2020Article
- Conditions of embryo culture from days 5 to 7 of development alter the DNA methylome of the bovine fetus at day 86 of gestation.Journal of assisted reproduction and genetics · 2020Article
- Determinants of survival of the bovine blastocyst to cryopreservation stress: treatment with colony stimulating factor 2 during the morula-to-blastocyst transition and embryo sex.CABI agriculture and bioscience · 2020Article
- Regulation of present and future development by maternal regulatory signals acting on the embryo during the morula to blastocyst transition - insights from the cow.Biology of reproduction · 2019Review
- Dickkopf-related protein 1 is a progestomedin acting on the bovine embryo during the morula-to-blastocyst transition to program trophoblast elongation.Scientific reports · 2019Article
- Embryo and cow factors affecting pregnancy per embryo transfer for multiple-service, lactating Holstein recipients.Translational animal science · 2019Article
- Sex affects immunolabeling for histone 3 K27me3 in the trophectoderm of the bovine blastocyst but not labeling for histone 3 K18ac.PloS one · 2019Article
- Follistatin supplementation during in vitro embryo culture improves developmental competence of bovine embryos produced using sex-sorted semen.Reproductive biology · 2018Article
- Effects of sex on response of the bovine preimplantation embryo to insulin-like growth factor 1, activin A, and WNT7A.BMC developmental biology · 2018Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We tested whether gene expression of the bovine morula is modified by CSF2 in a sex-dependent manner and if sex determines the effect of CSF2 on competence of embryos to become blastocysts. Embryos were produced in vitro using X- or Y-sorted semen and treated at Day 5 of culture with 10 ng/mL bovine CSF2 or control. In experiment 1, morulae were collected at Day 6 and biological replicates (n = 8) were evaluated for transcript abundance of 90 genes by RT-qPCR using the Fluidigm Delta Gene assay. Expression of more than one-third (33 of 90) of genes examined was affected by sex. The effect of CSF2 on gene expression was modified by sex (P < 0.05) for five genes (DDX3Y/DDX3X-like, NANOG, MYF6, POU5F1 and RIPK3) and tended (P < 0.10) to be modified by sex for five other genes (DAPK1, HOXA5, PPP2R3A, PTEN and TNFSF8). In experiment 2, embryos were treated at Day 5 with control or CSF2 and blastocysts were collected at Day 7 for immunolabeling to determine the number of inner cell mass (ICM) and trophectoderm (TE) cells. CSF2 increased the percent of putative zygotes that became blastocysts for females, but did not affect the development of males. There was no effect of CSF2 or interaction of CSF2 with sex on the total number of blastomeres in blastocysts or in the number of inner cell mass or trophectoderm cells. In conclusion, CSF2 exerted divergent responses on gene expression and development of female and male embryos. These results are evidence of sexually dimorphic responses of the preimplantation embryo to this embryokine.
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