ArticleProceedings of the National Academy of Sciences of the United States of America2016
Point mutations in the major outer membrane protein drive hypervirulence of a rapidly expanding clone of Campylobacter jejuni.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
38 citing papers in PubMed, 62 citations in OpenAlex.
- Article
- Sequence variability of BamA and FadL candidate vaccinogens suggests divergent evolutionary paths ofJournal of bacteriology · 2025Article
- Identification and characterization of an invasive, hyper-aerotolerantASM case reports · 2025Article
- Article
- First-ever ASM Global Research Symposium on Microbes in Human Health in Tsinghua, Beijing, China.mSphere · 2025Article
- Small RNA CjNC110 regulates the activated methyl cycle to enable optimal chicken colonization bymSphere · 2025Article
- Gene expression profile of Campylobacter jejuni in response to macrolide antibiotics.Archives of microbiology · 2024Article
- Article
- Recurrent Campylobacter jejuni Infections withMicrobiology spectrum · 2023Article
- Development of mass allelic exchange, a technique to enable sexual genetics inProceedings of the National Academy of Sciences of the United States of America · 2022Article
- Polar localization of CheO under hypoxia promotes Campylobacter jejuni chemotactic behavior within host.PLoS pathogens · 2022Article
- Article
- Ferric quinate (QPLEX) interacts with the major outer membrane protein (MOMP) ofComputational and structural biotechnology journal · 2022Article
- Structural Modeling of the Treponema pallidum Outer Membrane Protein Repertoire: a Road Map for Deconvolution of Syphilis Pathogenesis and Development of a Syphilis Vaccine.Journal of bacteriology · 2021Article
- Relationships of capsular polysaccharides belonging to Campylobacter jejuni HS1 serotype complex.PloS one · 2021Article
- Culture-free genome-wide locus sequence typing (GLST) provides new perspectives on Trypanosoma cruzi dispersal and infection complexity.PLoS genetics · 2020Article
- A Faster and More Accurate Algorithm for Calculating Population Genetics Statistics Requiring Sums of Stirling Numbers of the First Kind.G3 (Bethesda, Md.) · 2020Article
- Core Genome Multilocus Sequence Typing for Food Animal Source Attribution of HumanPathogens (Basel, Switzerland) · 2020Article
- Small Noncoding RNA CjNC110 Influences Motility, Autoagglutination, AI-2 Localization, Hydrogen Peroxide Sensitivity, and Chicken Colonization in Campylobacter jejuni.Infection and immunity · 2020Article
- Whole-Genome Comparisons ofApplied and environmental microbiology · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Infections due to clonal expansion of highly virulent bacterial strains are clear and present threats to human and animal health. Association of genetic changes with disease is now a routine, but identification of causative mutations that enable disease remains difficult. Campylobacter jejuni is an important zoonotic pathogen transmitted to humans mainly via the foodborne route. C. jejuni typically colonizes the gut, but a hypervirulent and rapidly expanding clone of C. jejuni recently emerged, which is able to translocate across the intestinal tract, causing systemic infection and abortion in pregnant animals. The genetic basis responsible for this hypervirulence is unknown. Here, we developed a strategy, termed "directed genome evolution," by using hybridization between abortifacient and nonabortifacient strains followed by selection in an animal disease model and whole-genome sequence analysis. This strategy successfully identified SNPs in porA, encoding the major outer membrane protein, are responsible for the hypervirulence. Defined mutagenesis verified that these mutations were both necessary and sufficient for causing abortion. Furthermore, sequence analysis identified porA as the gene with the top genome-wide signal of adaptive evolution using Fu's Fs, a population genetic metric for recent population size changes, which is consistent with the recent expansion of clone "sheep abortion." These results identify a key virulence factor in Campylobacter and a potential target for the control of this zoonotic pathogen. Furthermore, this study provides general, unbiased experimental and computational approaches that are broadly applicable for efficient elucidation of disease-causing mutations in bacterial pathogens.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.