Evidence map›Paper›PMID 27601586›Full record

ArticleMolecular and cellular biology2016

Extracellular Signal-Regulated Kinase 2 and CHOP Restrict the Expression of the Growth Arrest-Specific p20K Lipocalin Gene to G0.

M J Erb, D Camacho, W Xie, B M Maslikowski, B Fielding, R Ghosh, F-A Poujade, M Athar, S Assee, L-E Mantella and 1 more

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular and cellular biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.3field-weighted citation impact, top 30% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 1 institution in 1 country.

M J ErbDepartment of Biology, McMaster University, Hamilton, Ontario, Canada.
D CamachoDepartment of Biology, McMaster University, Hamilton, Ontario, Canada.
W XieDepartment of Biology, McMaster University, Hamilton, Ontario, Canada.
B M MaslikowskiDepartment of Biology, McMaster University, Hamilton, Ontario, Canada.
B FieldingDepartment of Biology, McMaster University, Hamilton, Ontario, Canada.
R GhoshDepartment of Biology, McMaster University, Hamilton, Ontario, Canada.
F-A PoujadeDepartment of Biology, McMaster University, Hamilton, Ontario, Canada.
M AtharDepartment of Biology, McMaster University, Hamilton, Ontario, Canada.
S AsseeDepartment of Biology, McMaster University, Hamilton, Ontario, Canada.
L-E MantellaDepartment of Biology, McMaster University, Hamilton, Ontario, Canada.
P-A BédardDepartment of Biology, McMaster University, Hamilton, Ontario, Canada abedard@mcmaster.ca.
McMaster University · CA

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The activation of the growth arrest-specific (gas) p20K gene depends on the interaction of C/EBPβ with two elements of a 48-bp promoter region termed the quiescence-responsive unit (QRU). Here we identify extracellular signal-related kinase 2 (ERK2) as a transcriptional repressor of the p20K QRU in cycling chicken embryo fibroblasts (CEF). ERK2 binds to repeated GAAAG sequences overlapping the C/EBPβ sites of the QRU. The recruitment of ERK2 and C/EBPβ is mutually exclusive and dictates the expression of p20K. C/EBP homologous protein (CHOP) was associated with C/EBPβ under conditions promoting endoplasmic reticulum (ER) stress and, to a lesser extent, in cycling CEF but was not detectable when C/EBPβ was immunoprecipitated from contact-inhibited cells. During ER stress, overexpression of CHOP inhibited p20K, while its downregulation promoted p20K, indicating that CHOP is also a potent inhibitor of p20K. Transcriptome analyses revealed that hypoxia-responsive genes are strongly induced in contact-inhibited but not serum-starved CEF, and elevated levels of nitroreductase activity, a marker of hypoxia, were detected at confluence. Conditions of hypoxia (2% O

Indexed as

AnimalsAvian ProteinsCCAAT-Enhancer-Binding Protein-betaCell CycleCell HypoxiaCell ProliferationCells, CulturedChick EmbryoEndoplasmic Reticulum StressFibroblastsGene Expression ProfilingGene Expression RegulationLipocalinsMitogen-Activated Protein Kinase 1Promoter Regions, GeneticTranscription Factor CHOPAvian ProteinsCCAAT-Enhancer-Binding Protein-betaLipocalinsMitogen-Activated Protein Kinase 1Transcription Factor CHOP

Identifiers

PMID27601586
PMCPMC5108878
OpenAlexW2550827727

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.