ReviewGenes & development2016
What have single-molecule studies taught us about gene expression?
Review in Genes & development, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed.
- Real-time single-molecule imaging of transcriptional regulatory networks in living cells.Nature reviews. Genetics · 2024Review
- Understanding Cell Differentiation Through Single-Cell Approaches: Conceptual Challenges of the Systemic Approach.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Recent Advances in Single-Molecule Tracking and Imaging Techniques.Annual review of analytical chemistry (Palo Alto, Calif.) · 2023Review
- Role of FcγRIII in the nasal cavity of BALB/c mice in the primary amebic meningoencephalitis protection model.Parasitology research · 2023Article
- Transcriptional activators in the early Drosophila embryo perform different kinetic roles.Cell systems · 2023Article
- Transcriptional enhancers at 40: evolution of a viral DNA element to nuclear architectural structures.Trends in genetics : TIG · 2022Review
- Article
- Dynamics of RNA polymerase II and elongation factor Spt4/5 recruitment during activator-dependent transcription.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Covalent labeling of nucleic acids.Chemical Society reviews · 2020Review
- Unraveling the molecular interactions involved in phase separation of glucocorticoid receptor.BMC biology · 2020Article
- Gene Networks with Transcriptional Bursting Recapitulate Rare Transient Coordinated High Expression States in Cancer.Cell systems · 2020Article
- A Mechanochemical Model of Transcriptional Bursting.Biophysical journal · 2020Article
- Nucleosomal proofreading of activator-promoter interactions.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Understanding biochemical processes in the presence of sub-diffusive behavior of biomolecules in solution and living cells.Biophysical reviews · 2019Review
- Modes of phase separation affecting chromatin regulation.Open biology · 2019Review
- ELTA: Enzymatic Labeling of Terminal ADP-Ribose.Molecular cell · 2019Article
- Transcription-Dependent Formation of Nuclear Granules Containing FUS and RNA Pol II.Biochemistry · 2018Article
- Assessing sufficiency and necessity of enhancer activities for gene expression and the mechanisms of transcription activation.Genes & development · 2018Review
- Dynamic enhancer function in the chromatin context.Wiley interdisciplinary reviews. Systems biology and medicine · 2018Review
- Single mRNA Molecule Detection in Drosophila.Methods in molecular biology (Clifton, N.J.) · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
Abstract
The production of a single mRNA is the result of many sequential steps, from docking of transcription factors to polymerase initiation, elongation, splicing, and, finally, termination. Much of our knowledge about the fundamentals of RNA synthesis and processing come from ensemble in vitro biochemical measurements. Single-molecule approaches are very much in this same reductionist tradition but offer exquisite sensitivity in space and time along with the ability to observe heterogeneous behavior and actually manipulate macromolecules. These techniques can also be applied in vivo, allowing one to address questions in living cells that were previously restricted to reconstituted systems. In this review, we examine the unique insights that single-molecule techniques have yielded on the mechanisms of gene expression.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.