ArticlePlant physiology2016
Primary Metabolism during Biosynthesis of Secondary Wall Polymers of Protoxylem Vessel Elements.
Article in Plant physiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed.
- Comprehensive analysis of SDH genes in Populus and functional characterization of SDH4 in xylem and cambium development.BMC plant biology · 2025Article
- Salicylic acid-mediated competitive regulation of xylem vessel formation and defense responses in Arabidopsis thaliana.Plant & cell physiology · 2025Article
- The Carbon Flow Shifts from Primary to Secondary Metabolism during Xylem Vessel Cell Differentiation in Arabidopsis thaliana.Plant & cell physiology · 2023Article
- Secondary cell wall patterning-connecting the dots, pits and helices.Open biology · 2022Article
- Hechtian Strands Transmit Cell Wall Integrity Signals in Plant Cells.Plants (Basel, Switzerland) · 2020Article
- MYB20, MYB42, MYB43, and MYB85 Regulate Phenylalanine and Lignin Biosynthesis during Secondary Cell Wall Formation.Plant physiology · 2020Article
- Differentiation of Tracheary Elements in Sugarcane Suspension Cells Involves Changes in Secondary Wall Deposition and Extensive Transcriptional Reprogramming.Frontiers in plant science · 2020Article
- Integrative omics of Lonicera japonica Thunb. Flower development unravels molecular changes regulating secondary metabolites.Journal of proteomics · 2019Article
- Xylan in the Middle: Understanding Xylan Biosynthesis and Its Metabolic Dependencies Toward Improving Wood Fiber for Industrial Processing.Frontiers in plant science · 2019Review
- Creating vessel elements in vitro: Towards a comprehensive understanding of the molecular basis of xylem vessel element differentiation.Plant biotechnology (Tokyo, Japan) · 2019Article
- Arabidopsis Group IIId ERF proteins positively regulate primary cell wall-type CESA genes.Journal of plant research · 2019Article
- Patterned Deposition of Xylan and Lignin is Independent from that of the Secondary Wall Cellulose of Arabidopsis Xylem Vessels.The Plant cell · 2018Article
- Proteomic analysis of xylem vessel cell differentiation in VND7-inducible tobacco BY-2 cells by two-dimensional gel electrophoresis.Plant biotechnology (Tokyo, Japan) · 2018Article
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13 authors.
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Abstract
Xylem vessels, the water-conducting cells in vascular plants, undergo characteristic secondary wall deposition and programmed cell death. These processes are regulated by the VASCULAR-RELATED NAC-DOMAIN (VND) transcription factors. Here, to identify changes in metabolism that occur during protoxylem vessel element differentiation, we subjected tobacco (Nicotiana tabacum) BY-2 suspension culture cells carrying an inducible VND7 system to liquid chromatography-mass spectrometry-based wide-target metabolome analysis and transcriptome analysis. Time-course data for 128 metabolites showed dynamic changes in metabolites related to amino acid biosynthesis. The concentration of glyceraldehyde 3-phosphate, an important intermediate of the glycolysis pathway, immediately decreased in the initial stages of cell differentiation. As cell differentiation progressed, specific amino acids accumulated, including the shikimate-related amino acids and the translocatable nitrogen-rich amino acid arginine. Transcriptome data indicated that cell differentiation involved the active up-regulation of genes encoding the enzymes catalyzing fructose 6-phosphate biosynthesis from glyceraldehyde 3-phosphate, phosphoenolpyruvate biosynthesis from oxaloacetate, and phenylalanine biosynthesis, which includes shikimate pathway enzymes. Concomitantly, active changes in the amount of fructose 6-phosphate and phosphoenolpyruvate were detected during cell differentiation. Taken together, our results show that protoxylem vessel element differentiation is associated with changes in primary metabolism, which could facilitate the production of polysaccharides and lignin monomers and, thus, promote the formation of the secondary cell wall. Also, these metabolic shifts correlate with the active transcriptional regulation of specific enzyme genes. Therefore, our observations indicate that primary metabolism is actively regulated during protoxylem vessel element differentiation to alter the cell's metabolic activity for the biosynthesis of secondary wall polymers.
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