ArticleVeterinary research2016
Two outer membrane proteins are bovine lactoferrin-binding proteins in Mannheimia haemolytica A1.
Article in Veterinary research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 28 citations in OpenAlex.
- Antigenicity analysis of the recombinant fusion proteins NlpI of Mannheimia haemolytica and DsbA of Pasteurella multocida.BMC veterinary research · 2025Article
- Antigenicity analysis of the recombinant outer membrane NlpI protein of Mannheimia haemolytica.Scientific reports · 2025Article
- Lactoferrin Affects the Viability of Bacteria in a Biofilm and the Formation of a New Biofilm Cycle ofInternational journal of molecular sciences · 2024Article
- Apo-Lactoferrin Inhibits the Proteolytic Activity of the 110 kDa Zn Metalloprotease Produced byInternational journal of molecular sciences · 2024Article
- Identification, Biochemical Characterization, and In Vivo Detection of a Zn-Metalloprotease with Collagenase Activity fromInternational journal of molecular sciences · 2024Article
- Phenotypic and genotypic assessment of iron acquisition in diverse bovine-associated non-aureus staphylococcal strains.Veterinary research · 2024Article
- Article
- Bovine apo-lactoferrin affects the secretion of proteases inAccess microbiology · 2021Article
- Lactoferrin and Its Derived Peptides: An Alternative for Combating Virulence Mechanisms Developed by Pathogens.Molecules (Basel, Switzerland) · 2020Review
- Differences between predicted outer membrane proteins of genotype 1 and 2 Mannheimia haemolytica.BMC microbiology · 2020Article
- Effect of apo-lactoferrin on leukotoxin and outer membrane vesicles of Mannheimia haemolytica A2.Veterinary research · 2020Article
- Synergistic Killing of Pathogenic Escherichia coli Using Camel Lactoferrin from Different Saudi Camel Clans and Various Antibiotics.The protein journal · 2019Article
- Lactoferrin: Balancing Ups and Downs of Inflammation Due to Microbial Infections.International journal of molecular sciences · 2017Review
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mannheimia haemolytica is a Gram negative bacterium that is part of the bovine respiratory disease, which causes important economic losses in the livestock industry. In the present work, the interaction between M. haemolytica A1 and bovine lactoferrin (BLf) was studied. This iron-chelating glycoprotein is part of the mammalian innate-immune system and is present in milk and mucosal secretions; Lf is also contained in neutrophils secondary granules, which release this glycoprotein at infection sites. It was evidenced that M. haemolytica was not able to use iron-charged BLf (BholoLf) as a sole iron source; nevertheless, iron-lacked BLf (BapoLf) showed a bactericidal effect against M. haemolytica with MIC of 4.88 ± 1.88 and 7.31 ± 1.62 μM for M. haemolytica strain F (field isolate) and M. haemolytica strain R (reference strain), respectively. Through overlay assays and 2-D electrophoresis, two OMP of 32.9 and 34.2 kDa with estimated IP of 8.18 and 9.35, respectively, were observed to bind both BapoLf and BholoLf; these OMP were identified by Maldi-Tof as OmpA (heat-modifiable OMP) and a membrane protein (porin). These M. haemolytica BLf binding proteins could be interacting in vivo with both forms of BLf depending on the iron state of the bovine.
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