Evidence map›Paper›PMID 27599857›Full record

ArticleActa crystallographica. Section F, Structural biology communications2016

Surface-layer protein from Caulobacter crescentus: expression, purification and X-ray crystallographic analysis.

Michael D Jones, Anson C K Chan, John F Nomellini, Michael E P Murphy, John Smit

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Acta crystallographica. Section F, Structural biology communications, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.5field-weighted citation impact, top 35% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 4 citations in OpenAlex.

  1. A bacterial surface layer protein exploits multistep crystallization for rapid self-assembly.Proceedings of the National Academy of Sciences of the United States of America · 2020
    Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Michael D JonesDepartment of Microbiology and Immunology, University of British Columbia, Vancouver, BC V6T 1Z3, Canada.
Anson C K ChanDepartment of Microbiology and Immunology, University of British Columbia, Vancouver, BC V6T 1Z3, Canada.
John F NomelliniDepartment of Microbiology and Immunology, University of British Columbia, Vancouver, BC V6T 1Z3, Canada.
Michael E P MurphyDepartment of Microbiology and Immunology, University of British Columbia, Vancouver, BC V6T 1Z3, Canada.
John SmitDepartment of Microbiology and Immunology, University of British Columbia, Vancouver, BC V6T 1Z3, Canada.
University of British Columbia · CA

Funding

CIHR
6 · The paper itself

Abstract

Protein surface layers are self-assembling, paracrystalline lattices on the surface of many prokaryotes. Surface-layer proteins have not benefited from widespread structural analysis owing to their resistance to crystallization. Here, the successful expression of a truncated version of RsaA, the surface-layer protein from Caulobacter crescentus, from a Caulobacter protein-expression system is reported. The purification, crystallization and initial X-ray diffraction analysis of the truncated RsaA, the largest surface-layer protein studied to date and the first from a Gram-negative bacterium, are also reported.

Indexed as

Bacterial Outer Membrane ProteinsCaulobacter crescentusCrystallizationCrystallography, X-RayGene ExpressionMembrane GlycoproteinsPlasmidsRecombinant ProteinsX-Ray DiffractionBacterial Outer Membrane ProteinsMembrane GlycoproteinsRecombinant ProteinsS-layer proteinsGram-negativephasingRTX motifS-layersurface-layer protein

Identifiers

PMID27599857
PMCPMC5012206
OpenAlexW2509805166

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.