ArticleScientific reports2016
Dual-color plasmonic enzyme-linked immunosorbent assay based on enzyme-mediated etching of Au nanoparticles.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 55 citations in OpenAlex.
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- A spectroscopy based prototype for the noninvasive detection of diabetes from human saliva using nanohybrids acting as nanozyme.Scientific reports · 2023Article
- An Electrochemical Immunosensor Based on Carboxylated Graphene/SPCE for IgG-SARS-CoV-2 Nucleocapsid Determination.Biosensors · 2022Article
- Shape dependent sensing potential of gold nanoparticles in etching based multicolorimetric plasmonic-ELISA.Nanoscale advances · 2022Article
- Entrapment of horseradish peroxidase into nanometer-scale metal-organic frameworks: a new nanocarrier for signal amplification in enzyme-linked immunosorbent assay.Mikrochimica acta · 2021Article
- A magnetic immunoconjugate nanoplatform for easy colorimetric detection of the NS1 protein of dengue virus in infected serum.Nanoscale advances · 2020Article
- Visual assay for determination of copper ions based on anti-etching of gold nanorods induced by cuprous ions.Mikrochimica acta · 2020Article
- Plasmonics for Biosensing.Materials (Basel, Switzerland) · 2019Review
- Development and Validation of a Reproducible and Label-Free Surface Plasmon Resonance Immunosensor for Enrofloxacin Detection in Animal-Derived Foods.Sensors (Basel, Switzerland) · 2017Article
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Colorimetric enzyme-linked immunosorbent assay utilizing 3'-3-5'-5-tetramethylbenzidine(TMB) as the chromogenic substrate has been widely used in the hospital for the detection of all kinds of disease biomarkers. Herein, we demonstrate a strategy to change this single-color display into dual-color responses to improve the accuracy of visual inspection. Our investigation firstly reveals that oxidation state of 3'-3-5'-5-tetramethylbenzidine (TMB(2+)) can quantitatively etch gold nanoparticles. Therefore, the incorporation of gold nanoparticles into a commercial TMB-based ELISA kit could generate dual-color responses: the solution color varied gradually from wine red (absorption peak located at ~530 nm) to colorless, and then from colorless to yellow (absorption peak located at ~450 nm) with the increase amount of targets. These dual-color responses effectively improved the sensitivity as well as the accuracy of visual inspection. For example, the proposed dual-color plasmonic ELISA is demonstrated for the detection of prostate-specific antigen (PSA) in human serum with a visual limit of detection (LOD) as low as 0.0093 ng/mL.
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