ArticlePloS one2016
Transcriptomic Changes Associated with Pregnancy in a Marsupial, the Gray Short-Tailed Opossum Monodelphis domestica.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
17 citing papers in PubMed, 1 synthesis or guideline pooled it, 40 citations in OpenAlex.
- Molecular Mechanism and Pathways of Normal Human Parturition in Different Gestational Tissues: A Systematic Review of Transcriptome Studies.Frontiers in physiology · 2021Pooled it
- Adaptive Immunity in Marsupials.Journal of experimental zoology. Part A, Ecological and integrative physiology · 2026Review
- Comparative Analysis of Progesterone Secretion and Plasma Proteome Across the Pregnant and Non-Pregnant Luteal Phase of the Koala (Proteomes · 2026Article
- Gene Expression and Alternative Splicing Throughout the Reproductive Cycle of a Viviparous Lizard Reveal Novel Genes for Pregnancy and Convergence With Squamates and Mammals.Molecular ecology · 2026Article
- Human embryo implantation.Development (Cambridge, England) · 2023Review
- Embryonic specializations for vertebrate placentation.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2022Review
- Integrated analyses of miRNA-mRNA expression profiles of ovaries reveal the crucial interaction networks that regulate the prolificacy of goats in the follicular phase.BMC genomics · 2021Article
- Identification of regenerative processes in neonatal spinal cord injury in the opossum (Monodelphis domestica): A transcriptomic study.The Journal of comparative neurology · 2021Article
- Evolutionary transcriptomics implicateseLife · 2021Article
- Uterine epithelial remodelling during pregnancy in the marsupial Monodelphis domestica (Didelphidae): Implications for mammalian placental evolution.Journal of anatomy · 2020Article
- Evidence for regulation of the complement system during pregnancy being ancient and conserved in mammals.Developmental and comparative immunology · 2020Article
- Cooperative inflammation: The recruitment of inflammatory signaling in marsupial and eutherian pregnancy.Journal of reproductive immunology · 2020Review
- Didelphis albiventris: an overview of unprecedented transcriptome sequencing of the white-eared opossum.BMC genomics · 2019Article
- Endometrial recognition of pregnancy occurs in the grey short-tailed opossum ( Monodelphis domestica).Proceedings. Biological sciences · 2019Article
- Transcriptomic changes in the pre-implantation uterus highlight histotrophic nutrition of the developing marsupial embryo.Scientific reports · 2018Article
- A pronounced uterine pro-inflammatory response at parturition is an ancient feature in mammals.Proceedings. Biological sciences · 2017Article
- Embryo implantation evolved from an ancestral inflammatory attachment reaction.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Live birth has emerged as a reproductive strategy many times across vertebrate evolution; however, mammals account for the majority of viviparous vertebrates. Marsupials are a mammalian lineage that last shared a common ancestor with eutherians (placental mammals) over 148 million years ago. Marsupials are noted for giving birth to highly altricial young after a short gestation, and represent humans' most distant viviparous mammalian relatives. Here we ask what insight can be gained into the evolution of viviparity in mammals specifically and vertebrates in general by analyzing the global uterine transcriptome in a marsupial. Transcriptome analyses were performed using NextGen sequencing of uterine RNA samples from the gray short-tailed opossum, Monodelphis domestica. Samples were collected from late stage pregnant, virgin, and non-pregnant experienced breeders. Three different algorithms were used to determine differential expression, and results were confirmed by quantitative PCR. Over 900 opossum gene transcripts were found to be significantly more abundant in the pregnant uterus than non-pregnant, and over 1400 less so. Most with increased abundance were genes related to metabolism, immune systems processes, and transport. This is the first study to characterize the transcriptomic differences between pregnant, non-pregnant breeders, and virgin marsupial uteruses and helps to establish a set of pregnancy-associated genes in the opossum. These observations allowed for comparative analyses of the differentially transcribed genes with other mammalian and non-mammalian viviparous species, revealing similarities in pregnancy related gene expression over 300 million years of amniote evolution.
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Registered trials
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