ArticleSensors (Basel, Switzerland)2016
Characterization of the ER-Targeted Low Affinity Ca(2+) Probe D4ER.
Article in Sensors (Basel, Switzerland), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 37 citations in OpenAlex.
- The photosensitive endoplasmic reticulum-chloroplast contact site.Journal of microscopy · 2025Article
- Luminal transport through intact endoplasmic reticulum limits the magnitude of localized CaProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Vitamin K-dependent carboxylation regulates CaCell reports · 2023Article
- Maprotiline restores ER homeostasis and rescues neurodegeneration via Histamine Receptor H1 inhibition in retinal ganglion cells.Nature communications · 2022Article
- Simultaneous imaging of ER and cytosolic Ca2+ dynamics reveals long-distance ER Ca2+ waves in plants.Plant physiology · 2021Article
- Generation and Characterization of a New FRET-Based CaInternational journal of molecular sciences · 2021Article
- Peroxisomes contribute to intracellular calcium dynamics in cardiomyocytes and non-excitable cells.Life science alliance · 2021Article
- Mitochondrial CaCells · 2021Review
- Article
- Reduced reticulum-mitochondria CaBasic research in cardiology · 2020Article
- Review
- Live Mitochondrial or Cytosolic Calcium Imaging Using Genetically-encoded Cameleon Indicator in Mammalian Cells.Bio-protocol · 2020Article
- Intracellular Calcium Dysregulation by the Alzheimer's Disease-Linked Protein Presenilin 2.International journal of molecular sciences · 2020Review
- PSEN2 (presenilin 2) mutants linked to familial Alzheimer disease impair autophagy by altering CaAutophagy · 2019Article
- ALS-Associated SOD1(G93A) Decreases SERCA Pump Levels and Increases Store-Operated CaInternational journal of molecular sciences · 2019Article
- Genetically targeted fluorescent probes reveal dynamic calcium responses to adrenergic signaling in multiple cardiomyocyte compartments.The international journal of biochemistry & cell biology · 2019Article
- The Trans Golgi Region is a Labile Intracellular CaScientific reports · 2018Article
- Generation and validation of novel adeno-associated viral vectors for the analysis of CaScientific reports · 2017Article
- Exploring cells with targeted biosensors.The Journal of general physiology · 2017Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Calcium ion (Ca(2+)) is a ubiquitous intracellular messenger and changes in its concentration impact on nearly every aspect of cell life. Endoplasmic reticulum (ER) represents the major intracellular Ca(2+) store and the free Ca(2+) concentration ([Ca(2+)]) within its lumen ([Ca(2+)]ER) can reach levels higher than 1 mM. Several genetically-encoded ER-targeted Ca(2+) sensors have been developed over the last years. However, most of them are non-ratiometric and, thus, their signal is difficult to calibrate in live cells and is affected by shifts in the focal plane and artifactual movements of the sample. On the other hand, existing ratiometric Ca(2+) probes are plagued by different drawbacks, such as a double dissociation constant (Kd) for Ca(2+), low dynamic range, and an affinity for the cation that is too high for the levels of [Ca(2+)] in the ER lumen. Here, we report the characterization of a recently generated ER-targeted, Förster resonance energy transfer (FRET)-based, Cameleon probe, named D4ER, characterized by suitable Ca(2+) affinity and dynamic range for monitoring [Ca(2+)] variations within the ER. As an example, resting [Ca(2+)]ER have been evaluated in a known paradigm of altered ER Ca(2+) homeostasis, i.e., in cells expressing a mutated form of the familial Alzheimer's Disease-linked protein Presenilin 2 (PS2). The lower Ca(2+) affinity of the D4ER probe, compared to that of the previously generated D1ER, allowed the detection of a conspicuous, more clear-cut, reduction in ER Ca(2+) content in cells expressing mutated PS2, compared to controls.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.