ArticleInternational journal of molecular sciences2016
Evaluation of Anthelmintic Activity and Composition of Pumpkin (Cucurbita pepo L.) Seed Extracts-In Vitro and in Vivo Studies.
Article in International journal of molecular sciences, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 82 citations in OpenAlex.
- Multicomponent mineral-phytogenic supplementation reduces Ascaridia galli shedding and systemic mycotoxin biomarkers while improving health and productive performance in laying hens.Veterinary world · 2026Article
- Accelerating wound healing rates with Cucurbita pepo leaf extract loaded electrospun poly(methyl methacrylate)/Halloysite/Chitosan/CaCO₃ composite nanofibers through in vitro and in vivo assessments.Drug delivery and translational research · 2026Article
- Phytochemical composition and in vitro anthelmintic activity of Erigeron floribundus (asteraceae) on three-stages of Haemonchus contortus of small ruminants.Scientific reports · 2026Article
- The use of pumpkin seeds as an alternative anthelmintic to reduce parasite burden in naturally infected lambs.Translational animal science · 2026Article
- Data-Driven FTIR Spectroscopy for the Discrimination of Nectars.Molecules (Basel, Switzerland) · 2025Article
- Indigenous medicinal knowledge and therapeutic practices of the endangered Ongota/Birale of Southwest Ethiopia.Journal of ethnobiology and ethnomedicine · 2025Article
- Profiling HydrophilicPlants (Basel, Switzerland) · 2025Article
- Potential ofPharmaceuticals (Basel, Switzerland) · 2025Article
- Anthelmintic Potential and In Silico Studies of Ricinoleic Acid from the Seed Oil ofInternational journal of molecular sciences · 2025Article
- Pumpkin seed oil: unveiling its potential in controlling inflammation and pathogenicity during experimental trichinellosis.BMC veterinary research · 2024Article
- Evaluation of anthelmintic efficacy of ethanolic leaf extract of Juglans regia L. on Ascaridia galli: a comprehensive in vitro and in vivo study.Veterinary research communications · 2024Article
- Anthelmintic Activity of Saussurea costus (Falc.) Lipsch. Against Ascaridia galli, a Pathogenic Nematode in Poultry: In Vitro and In Vivo Studies.Acta parasitologica · 2024Article
- Medicinal plants as a source of antiparasitics: an overview of experimental studies.Pathogens and global health · 2023Article
- The Effects ofMicroorganisms · 2023Article
- Assessment ofMolecules (Basel, Switzerland) · 2022Article
- Selective Supercritical COMolecules (Basel, Switzerland) · 2022Article
- Evaluation of Anthelmintic and Anti-Inflammatory Activity of 1,2,4-Triazole Derivatives.Molecules (Basel, Switzerland) · 2022Article
- Seeds of Cucurbita maxima and Carica papaya are effective for controlling monogeneans in the gills of Leporinus macrocephalus.Revista brasileira de parasitologia veterinaria = Brazilian journal of veterinary parasitology : Orgao Oficial do Colegio Brasileiro de Parasitologia Veterinaria · 2022Article
- Emerging Anthelmintic Resistance in Poultry: Can Ethnopharmacological Approaches Offer a Solution?Frontiers in pharmacology · 2021Review
- Cucurbitaceae COld Peeling Extracts (CCOPEs) Protect Plants From Root-Knot Nematode Infections Through Induced Resistance and Nematicidal Effects.Frontiers in plant science · 2021Article
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
unlabelledA significant number of studies report growing resistance in nematodes thriving in both humans and livestock. This study was conducted to evaluate the in vitro and in vivo anthelmintic efficiency of Curcubita pepo (C. pepo) L. hot water extract (HWE), cold water extract (CWE) or ethanol extract (ETE) on two model nematodes: Caenorhabditis elegans (C. elegans) and Heligmosoides bakeri (H. bakeri).
methodsRaman, IR and LC-MS spectroscopy analyses were performed on the studied plant material to deliver qualitative and quantitative data on the composition of the obtained extracts: ETE, HWE and CWE. The in vitro activity evaluation showed an impact of C. pepo extracts on C. elegans and different developmental stages of H. bakeri. The following in vivo experiments on mice infected with H. bakeri confirmed inhibitory properties of the most active pumpkin extract selected by the in vitro study. All of the extracts were found to contain cucurbitine, aminoacids, fatty acids, and-for the first time-berberine and palmatine were identified. All C. pepo seed extracts exhibited a nematidicidal potential in vitro, affecting the survival of L1 and L2 H. bakeri larvae. The ETE was the strongest and demonstrated a positive effect on H. bakeri eggs hatching and marked inhibitory properties against worm motility, compared to a PBS control. No significant effects of pumpkin seed extracts on C. elegans integrity or motility were found. The EtOH extract in the in vivo studies showed anthelmintic properties against both H. bakeri fecal egg counts and adult worm burdens. The highest egg counts reduction was observed for the 8 g/kg dose (IC50 against H. bakeri = 2.43; 95% Cl = 2.01-2.94). A decrease in faecal egg counts (FEC) was accompanied by a significant reduction in worm burden of the treated mice compared to the control group.
conclusionsPumpkin seed extracts may be used to control of Gastrointestinal (G.I.) nematode infections. This relatively inexpensive alternative to the currently available chemotherapeutic should be considered as a novel drug candidate in the nearest future.
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