ArticleJournal of parasitology research2016
Could kDNA-PCR in Peripheral Blood Replace the Examination of Bone Marrow for the Diagnosis of Visceral Leishmaniasis?
Article in Journal of parasitology research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 10 citations in OpenAlex.
- Advancing diagnosis of feline Leishmania infantum infection through experimental insights.Scientific reports · 2025Article
- Comparison of Three Commonly Used Genetic Markers for Detection ofEthiopian journal of health sciences · 2021Article
- Tegumentary leishmaniasis diagnosis: what happened with MST (Montenegro Skin Test) in Brazil?Revista do Instituto de Medicina Tropical de Sao Paulo · 2019Article
- Mathematical models of amino acid panel for assisting diagnosis of children acute leukemia.Journal of translational medicine · 2019Article
- Serological and molecular tools to diagnose visceral leishmaniasis: 2-years' experience of a single center in Northern Italy.PloS one · 2017Article
- Unusual Clinical Manifestations ofIranian journal of parasitologyArticle
- Determination of genetic diversity ofTropical parasitologyArticle
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The aim of this study was to evaluate whether the molecular (kDNA-PCR) and parasitological diagnosis in peripheral blood (PB) could replace the invasive and painful bone marrow collection (BM) in the diagnosis of visceral leishmaniasis (VL). PB from suspected VL patients was evaluated by parasitological and molecular techniques using as the gold standard (GS) a combination of clinical, epidemiological, and immunochromatographic test (PB-rK39) results and parasitological examination of BM. Based on the GS, 38 samples from 32 patients were grouped: Group 1, 20 samples of VL cases, and Group 2, 18 samples of non-VL cases. In order to evaluate the parasitological and molecular techniques in PB, the samples were examined. From Group 1, PB kDNA-PCR was positive in 20 samples and in 19 of 20 in BM kDNA-PCR examination. However, the parasitological examination of buffy coat was insensitive, being able to detect only 4 cases from Group 1. All samples from Group 2 were negative. We concluded that, for the diagnosis of visceral leishmaniasis, the parasitological examination of peripheral blood was not useful; however, molecular diagnosis by kDNA-PCR, performed in peripheral blood, could be useful to replace the parasitological examination of bone marrow.
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Registered trials
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