ArticleBMC biotechnology2016
Knockdown of five trehalase genes using RNA interference regulates the gene expression of the chitin biosynthesis pathway in Tribolium castaneum.
Article in BMC biotechnology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
41 citing papers in PubMed, 89 citations in OpenAlex.
- Identification, expression profiling, and functional characterization of trehalase genes (OaTRE1 and OaTRE) in Oedaleus asiaticus (Orthoptera: Acridoidea).Journal of insect science (Online) · 2026Article
- Validamycin Inhibits the Reproductive Capacity ofInsects · 2026Article
- Effects of neem extract on Artemia franciscana: Insights from high-throughput transcriptomics and phenotypic analysis.PloS one · 2026Article
- Physiological Response ofInsects · 2025Article
- Review
- Effects of Trehalase on the Gene Expression of the Reproductive Regulation Pathway Network and Triglyceride Metabolism inInsects · 2025Article
- Effects of Orally Delivered Double-Stranded RNA ofInsects · 2025Article
- The dual role of the RR-2 cuticular protein gene in development and acaricide susceptibility of Panonychus citri (Acari: Tetranychidae).Experimental & applied acarology · 2025Article
- Comparative transcriptomic and metabolomics analysis of ovary in Nilaparvata lugens after trehalase inhibition.BMC genomics · 2025Article
- A taste of one's own medicine:Frontiers in immunology · 2025Article
- Article
- Chitinase inhibition induces transcriptional dysregulation altering ecdysteroid-mediated control ofiScience · 2024Article
- Article
- Validamycin Inhibits the Synthesis and Metabolism of Trehalose and Chitin in the Oriental Fruit Fly,Insects · 2023Article
- Trehalose-6-phosphate synthase regulates chitin synthesis inFrontiers in physiology · 2023Article
- RNA interference-mediated knockdown of genes involved in sugar transport and metabolism disrupts psyllidFrontiers in insect science · 2023Article
- Potential inhibitory effects of compounds ZK-PI-5 and ZK-PI-9 on trehalose and chitin metabolism inFrontiers in physiology · 2023Article
- Knockdown of Two Trehalase Genes by RNA Interference Is Lethal to the White-Backed PlanthopperBiomolecules · 2022Article
- Article
- C/EBPα RegulatesInsects · 2022Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundRNA interference is a very effective approach for studies on gene function and may be an efficient method for controlling pests. Trehalase is a key gene in the chitin biosynthesis pathway in insects. Five trehalase genes have been cloned in Tribolium castaneum, though it is not known whether the detailed functions of these trehalases can be targeted for pest control.
resultsThe functions of all five trehalase genes were studied using RNAi, and the most important results showed that the expression of all 12 genes decreased significantly from 12 to 72 h compared with the control groups, except GP1 at 72 h, when the expression of the TcTre2 gene was suppressed. The results also revealed different abnormal phenotypes, and the observed mortality rates ranged from 17 to 42 %. The qRT-PCR results showed that the expression of TPS, GS, two GP, CHS1a and CHS1b genes decreased significantly, while that of the CHS2 gene decreased or increased after RNAi after the five trehalases were silenced at 48 h. In addition, TPS gene expression decreased from 12 to 72 h after dsTcTre injection.
conclusionsThese results demonstrate that silencing of any individual trehalase gene, especially Tre1-4 and Tre2 gene can lead to moulting deformities and a high mortality rate through the regulation of gene expression in the chitin biosynthesis pathway and may be a potential approach for pest control in the future.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.