ArticleScientific reports2016
On the mobility, membrane location and functionality of mechanosensitive channels in Escherichia coli.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 29 citations in OpenAlex.
- Uncovering transcriptional processes in microbial communities adapted to differing saline conditions in salt-weathered historic buildings.Microbiome · 2026Article
- Identification of MscS as a Key L-Glutamate Exporter in Bacillus methanolicus.Microbial biotechnology · 2025Article
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- Thiopseudomonas acetoxidans sp. nov., an aerobic acetic and butyric acids oxidizer isolated from anaerobic fermentation liquid of food waste.Antonie van Leeuwenhoek · 2024Article
- Super-resolving microscopy reveals the localizations and movement dynamics of stressosome proteins in Listeria monocytogenes.Communications biology · 2023Article
- How Functional Lipids Affect the Structure and Gating of Mechanosensitive MscS-like Channels.International journal of molecular sciences · 2022Review
- Structural basis for mechanotransduction in a potassium-dependent mechanosensitive ion channel.Nature communications · 2022Article
- Article
- Charged pore-lining residues are required for normal channel kinetics in the eukaryotic mechanosensitive ion channel MSL1.Channels (Austin, Tex.) · 2020Article
- Single-molecule localization to study cytoskeletal structures, membrane complexes, and mechanosensors.Biophysical reviews · 2019Review
- Synthetic and genetic dimers as quantification ruler for single-molecule counting with PALM.Molecular biology of the cell · 2019Article
- Decreased Effective Macromolecular Crowding in Escherichia coli Adapted to Hyperosmotic Stress.Journal of bacteriology · 2019Article
- Connecting the Dots between Mechanosensitive Channel Abundance, Osmotic Shock, and Survival at Single-Cell Resolution.Journal of bacteriology · 2018Article
- Steric exclusion and protein conformation determine the localization of plasma membrane transporters.Nature communications · 2018Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bacterial mechanosensitive channels protect cells from structural damage during hypoosmotic shock. MscS, MscL and MscK are the most abundant channels in E. coli and arguably the most important ones in osmoprotection. By combining physiological assays with quantitative photo-activated localization microscopy (qPALM), we find an almost linear relationship between channel abundance and cell survival. A minimum of 100 MscL (or MscS) channels is needed for protection when a single type of channel is expressed. Under native-like conditions MscL, MscS as well as MscK distribute homogeneously over the cytoplasmic membrane and the lateral diffusion of the channels is in accordance with their relative protein mass. However, we observe cluster formation and a reduced mobility of MscL when the majority of the subunits of the pentameric channel contain the fluorescent mEos3.2 protein. These data provide new insights into the quantitative biology of mechanosensitive channels and emphasizes the need for care in analysing protein complexes even when the fluorescent tag has been optimized for monomeric behaviour.
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Registered trials
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