ArticleAngewandte Chemie (International ed. in English)2016
Detection of Isoforms Differing by a Single Charge Unit in Individual Cells.
Article in Angewandte Chemie (International ed. in English), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 26 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
26 citing papers in PubMed, 46 citations in OpenAlex.
- Single-Cell Protein Assays in Context: From 2D to 3D and In Situ Analysis.Annual review of analytical chemistry (Palo Alto, Calif.) · 2026Review
- In situ and real-time monitoring of intracellular activities in single live cells using a nanopore probe.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Reducing Cathodic Drift during Isoelectric Focusing Using Microscale Immobilized pH Gradient Gels.Analytical chemistry · 2024Article
- Programmed Cell-Death Mechanism Analysis Using Same-Cell, Multimode DNA and Proteoform Electrophoresis.ACS measurement science au · 2021Article
- Summit: Automated Analysis of Arrayed Single-Cell Gel Electrophoresis.SLAS technology · 2021Article
- Segmentation-based analysis of single-cell immunoblots.Electrophoresis · 2021Article
- Multiplexed Ion Beam Imaging Readout of Single-Cell Immunoblotting.Analytical chemistry · 2021Article
- Single-cell proteomic analysis.WIREs mechanisms of disease · 2021Review
- 3D projection electrophoresis for single-cell immunoblotting.Nature communications · 2020Article
- In-gel fluorescence detection by DNA polymerase elongation.APL bioengineering · 2020Article
- Rapid electrotransfer probing for improved detection sensitivity in in-gel immunoassays.Analytical methods : advancing methods and applications · 2020Article
- Article
- Strategies for Development of a Next-Generation Protein Sequencing Platform.Trends in biochemical sciences · 2020Review
- Multiplexed in-gel microfluidic immunoassays: characterizing protein target loss during reprobing of benzophenone-modified hydrogels.Scientific reports · 2019Article
- Protein diffusion from microwells with contrasting hydrogel domains.APL bioengineering · 2019Article
- Controlling Dispersion during Single-Cell Polyacrylamide-Gel Electrophoresis in Open Microfluidic Devices.Analytical chemistry · 2018Article
- Microparticle Delivery of Protein Markers for Single-Cell Western Blotting from Microwells.Small (Weinheim an der Bergstrasse, Germany) · 2018Article
- Single cell protein analysis for systems biology.Essays in biochemistry · 2018Review
- Quantitative and multiplex microRNA assays from unprocessed cells in isolated nanoliter well arrays.Lab on a chip · 2018Article
- Arrayed isoelectric focusing using photopatterned multi-domain hydrogels.Electrophoresis · 2018Article
Corrections and comments
- Erratum issued
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
To measure protein isoforms in individual mammalian cells, we report single-cell resolution isoelectric focusing (scIEF) and high-selectivity immunoprobing. Microfluidic design and photoactivatable materials establish the tunable pH gradients required by IEF and precisely control the transport and handling of each 17-pL cell lysate during analysis. The scIEF assay resolves protein isoforms with resolution down to a single-charge unit, including both endogenous cytoplasmic and nuclear proteins from individual mammalian cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.